TY - JOUR
T1 - Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense
AU - Sakaguchi, Yoshihiko
AU - Uchiyama, Jumpei
AU - Také, Akira
AU - Gotoh, Kazuyoshi
AU - Sakaguchi, Masakiyo
AU - Suzuki, Tomonori
AU - Yamamoto, Yumiko
AU - Hosomi, Koji
AU - Kohda, Tomoko
AU - Mukamoto, Masafumi
AU - Kozaki, Shunji
AU - Hayashi, Shunji
AU - Oguma, Keiji
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (C) (No. 19K07560) from the Ministry of Education, Culture, Sports, Science and Technology and Kurozumi Medical Foundation .
Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (C) (No. 19K07560) from the Ministry of Education, Culture, Sports, Science and Technology and Kurozumi Medical Foundation.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/12
Y1 - 2020/12
N2 - Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense.
AB - Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense.
KW - Anaerobe
KW - Botulinum neurotoxin type G
KW - Botulism
KW - Clostridium argentinense
KW - Plasmid
UR - http://www.scopus.com/inward/record.url?scp=85093969147&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85093969147&partnerID=8YFLogxK
U2 - 10.1016/j.anaerobe.2020.102281
DO - 10.1016/j.anaerobe.2020.102281
M3 - Article
C2 - 33059044
AN - SCOPUS:85093969147
SN - 1075-9964
VL - 66
JO - Anaerobe
JF - Anaerobe
M1 - 102281
ER -
