Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense

Yoshihiko Sakaguchi, Jumpei Uchiyama, Akira Také, Kazuyoshi Gotoh, Masakiyo Sakaguchi, Tomonori Suzuki, Yumiko Yamamoto, Koji Hosomi, Tomoko Kohda, Masafumi Mukamoto, Shunji Kozaki, Shunji Hayashi, Keiji Oguma

Research output: Contribution to journalArticlepeer-review


Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense.

Original languageEnglish
Article number102281
Publication statusPublished - Dec 2020


  • Anaerobe
  • Botulinum neurotoxin type G
  • Botulism
  • Clostridium argentinense
  • Plasmid

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases


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