Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma

Hidetsugu Tsujigiwa; Hitoshi Nagatsuka; Pwint Han Phuu; Mehmet Gunduz; Huat Siar Chong; Shinichiro Oida; Noriyuki Nagai

doi:10.1016/j.oraloncology.2005.04.005

Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Pwint Han Phuu, Mehmet Gunduz, Huat Siar Chong, Shinichiro Oida, Noriyuki Nagai

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.

Original language	English
Pages (from-to)	843-850
Number of pages	8
Journal	Oral Oncology
Volume	41
Issue number	8
DOIs	https://doi.org/10.1016/j.oraloncology.2005.04.005
Publication status	Published - Sept 2005

Keywords

AMGX
AMGY
Ameloblastoma
Amelogenin
In situ hybridization
RT-PCR

ASJC Scopus subject areas

Oral Surgery
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.oraloncology.2005.04.005

Cite this

@article{965fd60132324081b56d04d278c25e12,

title = "Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma",

abstract = "Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.",

keywords = "AMGX, AMGY, Ameloblastoma, Amelogenin, In situ hybridization, RT-PCR",

author = "Hidetsugu Tsujigiwa and Hitoshi Nagatsuka and Phuu, {Pwint Han} and Mehmet Gunduz and Chong, {Huat Siar} and Shinichiro Oida and Noriyuki Nagai",

note = "Funding Information: This study was supported by Grant-in-Aid for Scientific Research (A) from the Japanese Ministry of Education, Culture, Sports, Science and Technology (No. 1520960).",

year = "2005",

month = sep,

doi = "10.1016/j.oraloncology.2005.04.005",

language = "English",

volume = "41",

pages = "843--850",

journal = "Oral Oncology",

issn = "1368-8375",

publisher = "Elsevier Limited",

number = "8",

}

TY - JOUR

T1 - Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma

AU - Tsujigiwa, Hidetsugu

AU - Nagatsuka, Hitoshi

AU - Phuu, Pwint Han

AU - Gunduz, Mehmet

AU - Chong, Huat Siar

AU - Oida, Shinichiro

AU - Nagai, Noriyuki

N1 - Funding Information: This study was supported by Grant-in-Aid for Scientific Research (A) from the Japanese Ministry of Education, Culture, Sports, Science and Technology (No. 1520960).

PY - 2005/9

Y1 - 2005/9

N2 - Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.

AB - Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.

KW - AMGX

KW - AMGY

KW - Ameloblastoma

KW - Amelogenin

KW - In situ hybridization

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=23744491414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23744491414&partnerID=8YFLogxK

U2 - 10.1016/j.oraloncology.2005.04.005

DO - 10.1016/j.oraloncology.2005.04.005

M3 - Article

C2 - 15979380

AN - SCOPUS:23744491414

SN - 1368-8375

VL - 41

SP - 843

EP - 850

JO - Oral Oncology

JF - Oral Oncology

IS - 8

ER -

Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this