@inbook{62530529b1c84136b658f10f28f2a1a4,
title = "Analysis of expression of CCN family genes in skeletal tissue-derived cells",
abstract = "The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.",
keywords = "Chondrocyte, Collagens, Extracellular matrix (ECM), Matrix of bone, Matrix of cartilage, Mesenchymal cells, Osteoblast, Proteoglycans, RNA purification, Real-time PCR, qRT-PCR",
author = "Harumi Kawaki and Satoshi Kubota and Masaharu Takigawa",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_4",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "33--41",
booktitle = "Methods in Molecular Biology",
}