Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system

Shoji Sonoda; Hisaaki Tsumuki

doi:10.1007/s10327-003-0116-1

Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system

Shoji Sonoda, Hisaaki Tsumuki

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Virus interactions between Tomato spotted wilt virus (TSWV) and Potato virus X (PVX) containing the nucleocapsid protein (N) gene sequences were examined to evaluate the capacity of the N gene sequences from TSWV to promote RNA-mediated cross-protection. Plants simultaneously inoculated with TSWV and PVX containing the 3′ 96bp of the N gene were highly resistant to TSWV infection, whereas no such resistance was observed in plants inoculated with TSWV and PVX containing the 5′ 96 bp. These results suggest that the 3′ portion of the N gene has a higher capacity for promoting RNA-mediated cross-protection of TSWV.

Original language	English
Pages (from-to)	239-242
Number of pages	4
Journal	Journal of General Plant Pathology
Volume	70
Issue number	4
DOIs	https://doi.org/10.1007/s10327-003-0116-1
Publication status	Published - Aug 2004

Keywords

Cross-protection
Nicotiana benthamiana
RNA silencing
Tospovirus
Virus resistance

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-003-0116-1

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title = "Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system",

abstract = "Virus interactions between Tomato spotted wilt virus (TSWV) and Potato virus X (PVX) containing the nucleocapsid protein (N) gene sequences were examined to evaluate the capacity of the N gene sequences from TSWV to promote RNA-mediated cross-protection. Plants simultaneously inoculated with TSWV and PVX containing the 3′ 96bp of the N gene were highly resistant to TSWV infection, whereas no such resistance was observed in plants inoculated with TSWV and PVX containing the 5′ 96 bp. These results suggest that the 3′ portion of the N gene has a higher capacity for promoting RNA-mediated cross-protection of TSWV.",

keywords = "Cross-protection, Nicotiana benthamiana, RNA silencing, Tospovirus, Virus resistance",

author = "Shoji Sonoda and Hisaaki Tsumuki",

note = "Funding Information: Acknowledgment This study was supported in part by The Ohara Foundation for Agricultural Research and The Sanyo Broadcasting Foundation for Scientific Research. The authors thank Dr. D.C. Baulcombe (Sainsbury Laboratory, Norwich, UK) for providing the PVX vector; Dr. S. Tsuda (National Agricultural Research Center, Tsukuba, Japan) for providing Tomato spotted wilt virus; and Dr. M. Nishiguchi (Ehime University, Matsuyama, Japan) for reading the manuscript.",

year = "2004",

month = aug,

doi = "10.1007/s10327-003-0116-1",

language = "English",

volume = "70",

pages = "239--242",

journal = "Journal of General Plant Pathology",

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AU - Sonoda, Shoji

AU - Tsumuki, Hisaaki

N1 - Funding Information: Acknowledgment This study was supported in part by The Ohara Foundation for Agricultural Research and The Sanyo Broadcasting Foundation for Scientific Research. The authors thank Dr. D.C. Baulcombe (Sainsbury Laboratory, Norwich, UK) for providing the PVX vector; Dr. S. Tsuda (National Agricultural Research Center, Tsukuba, Japan) for providing Tomato spotted wilt virus; and Dr. M. Nishiguchi (Ehime University, Matsuyama, Japan) for reading the manuscript.

PY - 2004/8

Y1 - 2004/8

N2 - Virus interactions between Tomato spotted wilt virus (TSWV) and Potato virus X (PVX) containing the nucleocapsid protein (N) gene sequences were examined to evaluate the capacity of the N gene sequences from TSWV to promote RNA-mediated cross-protection. Plants simultaneously inoculated with TSWV and PVX containing the 3′ 96bp of the N gene were highly resistant to TSWV infection, whereas no such resistance was observed in plants inoculated with TSWV and PVX containing the 5′ 96 bp. These results suggest that the 3′ portion of the N gene has a higher capacity for promoting RNA-mediated cross-protection of TSWV.

AB - Virus interactions between Tomato spotted wilt virus (TSWV) and Potato virus X (PVX) containing the nucleocapsid protein (N) gene sequences were examined to evaluate the capacity of the N gene sequences from TSWV to promote RNA-mediated cross-protection. Plants simultaneously inoculated with TSWV and PVX containing the 3′ 96bp of the N gene were highly resistant to TSWV infection, whereas no such resistance was observed in plants inoculated with TSWV and PVX containing the 5′ 96 bp. These results suggest that the 3′ portion of the N gene has a higher capacity for promoting RNA-mediated cross-protection of TSWV.

KW - Cross-protection

KW - Nicotiana benthamiana

KW - RNA silencing

KW - Tospovirus

KW - Virus resistance

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Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system

Abstract

Keywords

ASJC Scopus subject areas

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