Analysis of progranulin-mediated Akt and MAPK activation

Shi Qiong Xu, Simone Buraschi, Ryuta Tanimoto, Manuela Stefanello, Antonino Belfiore, Renato V. Iozzo, Andrea Morrione

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)


Progranulin has emerged in recent years as an important regulator of various biological functions including cell proliferation, wound healing, motility, and protection from apoptosis. Progranulin is also critical for transformation as established in several cancer models. Progranulin biological responses elicit through the activation of the Akt and MAPK pathways, which are critical for progranulin downstream signaling. In this chapter various experimental approaches aiming at detecting progranulin-mediated Akt and MAPK activation will be discussed.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Akt
  • Confocal analysis
  • Electrophoresis
  • Immunofluorescence
  • MAP kinase
  • Nitrocellulose membrane
  • PathScan
  • Progranulin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Analysis of progranulin-mediated Akt and MAPK activation'. Together they form a unique fingerprint.

Cite this