@inbook{7b10ad4fe04442b897bbc33e17af0b92,
title = "Analysis of progranulin-mediated Akt and MAPK activation",
abstract = "Progranulin has emerged in recent years as an important regulator of various biological functions including cell proliferation, wound healing, motility, and protection from apoptosis. Progranulin is also critical for transformation as established in several cancer models. Progranulin biological responses elicit through the activation of the Akt and MAPK pathways, which are critical for progranulin downstream signaling. In this chapter various experimental approaches aiming at detecting progranulin-mediated Akt and MAPK activation will be discussed.",
keywords = "Akt, Confocal analysis, Electrophoresis, Immunofluorescence, MAP kinase, Nitrocellulose membrane, PathScan, Progranulin, SDS PAGE",
author = "Xu, {Shi Qiong} and Simone Buraschi and Ryuta Tanimoto and Manuela Stefanello and Antonino Belfiore and Iozzo, {Renato V.} and Andrea Morrione",
note = "Funding Information: Our laboratories are supported by the Benjamin Perkins Bladder Cancer Fund, the Schorsch Family Fund for Innovative Medical Research and Care, National Institutes of Health Grants RO1 CA164462 (A.M., R.V.I.), Associazione Italiana per la Ricerca sul Cancro (AIRC) (grant n. 10625/12), AIRC project Calabria 2015, and Fondazione Cassa di Risparmio di Calabria e Lucania PON01_01078 (A.B.). Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8559-3_9",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "121--130",
booktitle = "Methods in Molecular Biology",
}
