Angiogenic activity of osteopontin-derived peptide SVVYGLR

Yoshinosuke Hamada, Kiyoshi Nokihara, Masayuki Okazaki, Wataru Fujitani, Takuya Matsumoto, Mitsuyoshi Matsuo, Yukichi Umakoshi, Junzou Takahashi, Nariaki Matsuura

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)


Angiogenesis plays an important role in various pathological conditions as well as some physiological processes. Although a number of soluble angiogenic factors have been reported, extracellular matrix also has crucial effect on angiogenesis through interaction with endothelial cells. Since recent reports showed osteopontin had some angiogenic activity, the effect of the SVVYGLR peptide, novel binding motif in osteopontin molecule, on angiogenesis was examined in this study. Synthetic peptide SVVYGLR did not have proliferative effect on endothelial cells but adhesion and migration activity to endothelial cells. Furthermore, SVVYGLR had as potent activity for tube formation in three-dimensional collagen gel as vascular endothelial growth factor which is known to be the strongest angiogenic factor. Electron microscopical analysis showed a number of microvilli on the endothelial luminar surface and tight junction formation in the luminar intercellular border between endothelial cells, indicating SVVYGLR induced cell porarity and differentiation of endothelial cells. This small peptide might be expected to stimulate angiogenesis to improve some ischemic conditions in the future because of some advantages due to smaller molecular weight.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - Oct 10 2003
Externally publishedYes


  • Angiogenesis
  • Differentiation
  • Endothelial cells
  • Osteopontin
  • Tube formation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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