Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma

Manabu Onishi; Tomotsugu Ichikawa; Kazuhiko Kurozumi; Satoshi Inoue; Tomoko Maruo; Yoshihiro Otani; Kentaro Fujii; Joji Ishida; Yosuke Shimazu; Koichi Yoshida; Hiroyuki Michiue; E. Antonio Chiocca; Isao Date

doi:10.1007/s10014-015-0216-6

Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma

Manabu Onishi, Tomotsugu Ichikawa, Kazuhiko Kurozumi, Satoshi Inoue, Tomoko Maruo, Yoshihiro Otani, Kentaro Fujii, Joji Ishida, Yosuke Shimazu, Koichi Yoshida, Hiroyuki Michiue, E. Antonio Chiocca, Isao Date

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

We have established a pair of animal models (J3T-1 and J3T-2) with different invasive and angiogenic phenotypes, and demonstrated that annexin A2 is expressed at higher levels in J3T-1 than J3T-2 cells. The function of annexin A2 in relation to angiogenesis and invasion was investigated using these models. Stable silencing or overexpression of annexin A2 in J3T-1 and J3T-2 cells (J3T-1shA and J3T-2A cells) was established and used. Thirty human glioblastoma samples were evaluated for expression of annexin A2, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). Immunohistochemical and quantitative reverse-transcription polymerase chain reaction analyses revealed higher expression of annexin A2, VEGF and PDGF in J3T-1 and J3T-2A cells. Cultured J3T-1 and J3T-2A cells exhibited higher adhesive ability to endothelial cells. Histopathological analysis of animal brain tumors revealed that J3T-1 and J3T-2A tumors displayed marked angiogenesis and invasion along the neovasculature, whereas J3T-2 and J3T-1shA tumors exhibited diffuse, infiltrative invasion without angiogenesis. Positive expression of annexin A2 was observed in tumor cells surrounding dilated vessels in 25/30 human glioblastoma specimens. Our results reveal that the phenotype of glioma invasion is closely related to angiogenesis. We identify annexin A2 as a factor regulating angiogenesis and invasion of malignant gliomas.

Original language	English
Pages (from-to)	184-194
Number of pages	11
Journal	Brain tumor pathology
Volume	32
Issue number	3
DOIs	https://doi.org/10.1007/s10014-015-0216-6
Publication status	Published - Jul 25 2015

Keywords

Angiogenesis
Animal model
Annexin A2
Glioma
Invasion

ASJC Scopus subject areas

Oncology
Clinical Neurology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10014-015-0216-6

Cite this

@article{32f5a93cffc44c3ebe03f8d27d6e9ab0,

title = "Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma",

abstract = "We have established a pair of animal models (J3T-1 and J3T-2) with different invasive and angiogenic phenotypes, and demonstrated that annexin A2 is expressed at higher levels in J3T-1 than J3T-2 cells. The function of annexin A2 in relation to angiogenesis and invasion was investigated using these models. Stable silencing or overexpression of annexin A2 in J3T-1 and J3T-2 cells (J3T-1shA and J3T-2A cells) was established and used. Thirty human glioblastoma samples were evaluated for expression of annexin A2, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). Immunohistochemical and quantitative reverse-transcription polymerase chain reaction analyses revealed higher expression of annexin A2, VEGF and PDGF in J3T-1 and J3T-2A cells. Cultured J3T-1 and J3T-2A cells exhibited higher adhesive ability to endothelial cells. Histopathological analysis of animal brain tumors revealed that J3T-1 and J3T-2A tumors displayed marked angiogenesis and invasion along the neovasculature, whereas J3T-2 and J3T-1shA tumors exhibited diffuse, infiltrative invasion without angiogenesis. Positive expression of annexin A2 was observed in tumor cells surrounding dilated vessels in 25/30 human glioblastoma specimens. Our results reveal that the phenotype of glioma invasion is closely related to angiogenesis. We identify annexin A2 as a factor regulating angiogenesis and invasion of malignant gliomas.",

keywords = "Angiogenesis, Animal model, Annexin A2, Glioma, Invasion",

author = "Manabu Onishi and Tomotsugu Ichikawa and Kazuhiko Kurozumi and Satoshi Inoue and Tomoko Maruo and Yoshihiro Otani and Kentaro Fujii and Joji Ishida and Yosuke Shimazu and Koichi Yoshida and Hiroyuki Michiue and {Antonio Chiocca}, E. and Isao Date",

note = "Funding Information: We thank M. Arao, A. Ishikawa and N. Uemori for their technical assistance. The following medical students also contributed to the animal experiments: T. Mifune, S. Murai, M. Matsueda, H. Matsumoto and Y. Yoshida. This study was supported by Grants-in-aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology to T.I. (No. 19591675; No. 22591611) and K.K. (No. 20890133; No. 21791364). Publisher Copyright: {\textcopyright} 2015, The Japan Society of Brain Tumor Pathology.",

year = "2015",

month = jul,

day = "25",

doi = "10.1007/s10014-015-0216-6",

language = "English",

volume = "32",

pages = "184--194",

journal = "Brain Tumor Pathology",

issn = "1433-7398",

publisher = "Springer Japan",

number = "3",

}

TY - JOUR

T1 - Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma

AU - Onishi, Manabu

AU - Ichikawa, Tomotsugu

AU - Kurozumi, Kazuhiko

AU - Inoue, Satoshi

AU - Maruo, Tomoko

AU - Otani, Yoshihiro

AU - Fujii, Kentaro

AU - Ishida, Joji

AU - Shimazu, Yosuke

AU - Yoshida, Koichi

AU - Michiue, Hiroyuki

AU - Antonio Chiocca, E.

AU - Date, Isao

N1 - Funding Information: We thank M. Arao, A. Ishikawa and N. Uemori for their technical assistance. The following medical students also contributed to the animal experiments: T. Mifune, S. Murai, M. Matsueda, H. Matsumoto and Y. Yoshida. This study was supported by Grants-in-aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology to T.I. (No. 19591675; No. 22591611) and K.K. (No. 20890133; No. 21791364). Publisher Copyright: © 2015, The Japan Society of Brain Tumor Pathology.

PY - 2015/7/25

Y1 - 2015/7/25

N2 - We have established a pair of animal models (J3T-1 and J3T-2) with different invasive and angiogenic phenotypes, and demonstrated that annexin A2 is expressed at higher levels in J3T-1 than J3T-2 cells. The function of annexin A2 in relation to angiogenesis and invasion was investigated using these models. Stable silencing or overexpression of annexin A2 in J3T-1 and J3T-2 cells (J3T-1shA and J3T-2A cells) was established and used. Thirty human glioblastoma samples were evaluated for expression of annexin A2, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). Immunohistochemical and quantitative reverse-transcription polymerase chain reaction analyses revealed higher expression of annexin A2, VEGF and PDGF in J3T-1 and J3T-2A cells. Cultured J3T-1 and J3T-2A cells exhibited higher adhesive ability to endothelial cells. Histopathological analysis of animal brain tumors revealed that J3T-1 and J3T-2A tumors displayed marked angiogenesis and invasion along the neovasculature, whereas J3T-2 and J3T-1shA tumors exhibited diffuse, infiltrative invasion without angiogenesis. Positive expression of annexin A2 was observed in tumor cells surrounding dilated vessels in 25/30 human glioblastoma specimens. Our results reveal that the phenotype of glioma invasion is closely related to angiogenesis. We identify annexin A2 as a factor regulating angiogenesis and invasion of malignant gliomas.

AB - We have established a pair of animal models (J3T-1 and J3T-2) with different invasive and angiogenic phenotypes, and demonstrated that annexin A2 is expressed at higher levels in J3T-1 than J3T-2 cells. The function of annexin A2 in relation to angiogenesis and invasion was investigated using these models. Stable silencing or overexpression of annexin A2 in J3T-1 and J3T-2 cells (J3T-1shA and J3T-2A cells) was established and used. Thirty human glioblastoma samples were evaluated for expression of annexin A2, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). Immunohistochemical and quantitative reverse-transcription polymerase chain reaction analyses revealed higher expression of annexin A2, VEGF and PDGF in J3T-1 and J3T-2A cells. Cultured J3T-1 and J3T-2A cells exhibited higher adhesive ability to endothelial cells. Histopathological analysis of animal brain tumors revealed that J3T-1 and J3T-2A tumors displayed marked angiogenesis and invasion along the neovasculature, whereas J3T-2 and J3T-1shA tumors exhibited diffuse, infiltrative invasion without angiogenesis. Positive expression of annexin A2 was observed in tumor cells surrounding dilated vessels in 25/30 human glioblastoma specimens. Our results reveal that the phenotype of glioma invasion is closely related to angiogenesis. We identify annexin A2 as a factor regulating angiogenesis and invasion of malignant gliomas.

KW - Angiogenesis

KW - Animal model

KW - Annexin A2

KW - Glioma

KW - Invasion

UR - http://www.scopus.com/inward/record.url?scp=84937974710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937974710&partnerID=8YFLogxK

U2 - 10.1007/s10014-015-0216-6

DO - 10.1007/s10014-015-0216-6

M3 - Article

C2 - 25697644

AN - SCOPUS:84937974710

SN - 1433-7398

VL - 32

SP - 184

EP - 194

JO - Brain Tumor Pathology

JF - Brain Tumor Pathology

IS - 3

ER -

Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this