Antimicrobial susceptibility of clinical isolates of aerobic Gram-negative bacteria in 2004

Isamu Yoshida, Takaji Fujimura, Yoshihisa Itoh, Mineji Tachibana, Mitsuo Kaku, Keiji Kanemitsu, Choichiro Takahashi, Joji Shiotani, Yuka Ono, Hisashi Baba, Shuji Matsuo, Seishi Asari, Kimiko Matsuoka, Nobuchika Kusano, Motoko Nose, Tetsunori Saikawa, Kazufumi Hiramatsu, Shigeru Kohno, Yoichi Hirakata, Nobuhisa YamaneIsamu Nakasone, Yoshinori Yamano

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


We determined MICs of antibacterial agents against 1,248 clinical strains of aerobic Gram-negative bacteria (19 genus or species) isolated at 16 Japanese facilities in 2004. MICs were determined using mostly broth microdilution method and antibacterial activity was assessed. Antibacterial susceptibility of Enterobacteriaceae to most β-lactams was comparable to that described in our previous report on isolates in 2002. Strains producing extended-spectrum β-lactamases(ESBL) accounted for 2.5% of Escherichia coli, 0% of Klebsiella spp., and 9.2% of Proteus spp. Notably, 16.7% of Proteus mirabilis was found to produce ESBL. Most antibacterial agents showed good activity against Moraxella catarrhalis. Among Haemophilus influenzae, 9.3% produced β-lactamase and 57.7% were β-lactamase-negative ampicillin-resistant strains when classified by penicillin-binding protein 3 mutation. Although few antibacterial agents against Pseudomonas aeruginosa have potent activity, only three agents-tobramycin, doripenem, and amikacin-showed an MIC90 of ≤8 μg/mL. Of all P. aeruginosa strains, 8.9% were resistant to seven or more agents of ten antipseudomonal agents, a decrease compared to results reported in 2002. Against other glucose-non-fermentative Gram-negative bacteria, the activity of most antibacterial agents was similar to that in 2002.

Original languageEnglish
Pages (from-to)562-579
Number of pages18
JournalJapanese Journal of Chemotherapy
Issue number5
Publication statusPublished - Sept 2008
Externally publishedYes


  • Clinical isolate
  • Drug susceptibility
  • Gram-negative bacteria
  • Multidrug-resistant pseudomonas aeruginosa
  • Surveillance

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)


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