Antioxidative effects of a novel dietary supplement Neumentix in a mouse stroke model

Yuki Taira; Toru Yamashita; Yuting Bian; Jingwei Shang; Namiko Matsumoto; Ryo Sasaki; Koh Tadokoro; Emi Nomura; Keiichiro Tsunoda; Yosio Omote; Mami Takemoto; Nozomi Hishikawa; Yasuyuki Ohta; Koji Abe

doi:10.1016/j.jstrokecerebrovasdis.2020.104818

Antioxidative effects of a novel dietary supplement Neumentix in a mouse stroke model

Yuki Taira, Toru Yamashita, Yuting Bian, Jingwei Shang, Namiko Matsumoto, Ryo Sasaki, Koh Tadokoro, Emi Nomura, Keiichiro Tsunoda, Yosio Omote, Mami Takemoto, Nozomi Hishikawa, Yasuyuki Ohta, Koji Abe

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Background: During an acute stroke, reactive oxygen species are overproduced and the endogenous antioxidative defense systems are disrupted. Therefore, antioxidative therapy can be a promising scheme to reduce the severity of stroke. Neumentix is a novel antioxidative supplement produced from a patented mint line and contains a high content of rosmarinic acid (RA). Although Neumentix has proven diverse efficacy and safety in clinical trials, its effect on strokes is unclear. Methods: Mice that were treated with Neumentix or vehicle for 14 days underwent transient middle cerebral artery occlusion (tMCAO) for 60 min. Mice were sacrificed 5 days after tMCAO. Results: Neumentix preserved body weight after tMCAO, showed a high antioxidative effect in serum, and reduced infarction volume compared to the vehicle. The expression of 4-hydroxy-2-nonenal, Nε-(carboxymethyl) lysine, and 8-hydroxy-2′-deoxyguanosine was reduced in Neumentix-treated mice. Conclusion: The antioxidative effect of Neumentix was confirmed. This is the first report to demonstrate the antioxidative effect of Neumentix on strokes.

Original language	English
Article number	104818
Journal	Journal of Stroke and Cerebrovascular Diseases
Volume	29
Issue number	8
DOIs	https://doi.org/10.1016/j.jstrokecerebrovasdis.2020.104818
Publication status	Published - Aug 2020

Keywords

Infarction volume
Mint
ROS
Rosmarinic acid

ASJC Scopus subject areas

Surgery
Rehabilitation
Clinical Neurology
Cardiology and Cardiovascular Medicine

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10.1016/j.jstrokecerebrovasdis.2020.104818

Cite this

Taira, Y., Yamashita, T., Bian, Y., Shang, J., Matsumoto, N., Sasaki, R., Tadokoro, K., Nomura, E., Tsunoda, K., Omote, Y., Takemoto, M., Hishikawa, N., Ohta, Y., & Abe, K. (2020). Antioxidative effects of a novel dietary supplement Neumentix in a mouse stroke model. Journal of Stroke and Cerebrovascular Diseases, 29(8), Article 104818. https://doi.org/10.1016/j.jstrokecerebrovasdis.2020.104818

Taira, Y, Yamashita, T, Bian, Y, Shang, J, Matsumoto, N, Sasaki, R, Tadokoro, K, Nomura, E, Tsunoda, K, Omote, Y, Takemoto, M, Hishikawa, N, Ohta, Y & Abe, K 2020, 'Antioxidative effects of a novel dietary supplement Neumentix in a mouse stroke model', Journal of Stroke and Cerebrovascular Diseases, vol. 29, no. 8, 104818. https://doi.org/10.1016/j.jstrokecerebrovasdis.2020.104818

@article{fbaab783fc4c4070afb014d7f38d3c18,

title = "Antioxidative effects of a novel dietary supplement Neumentix in a mouse stroke model",

abstract = "Background: During an acute stroke, reactive oxygen species are overproduced and the endogenous antioxidative defense systems are disrupted. Therefore, antioxidative therapy can be a promising scheme to reduce the severity of stroke. Neumentix is a novel antioxidative supplement produced from a patented mint line and contains a high content of rosmarinic acid (RA). Although Neumentix has proven diverse efficacy and safety in clinical trials, its effect on strokes is unclear. Methods: Mice that were treated with Neumentix or vehicle for 14 days underwent transient middle cerebral artery occlusion (tMCAO) for 60 min. Mice were sacrificed 5 days after tMCAO. Results: Neumentix preserved body weight after tMCAO, showed a high antioxidative effect in serum, and reduced infarction volume compared to the vehicle. The expression of 4-hydroxy-2-nonenal, Nε-(carboxymethyl) lysine, and 8-hydroxy-2′-deoxyguanosine was reduced in Neumentix-treated mice. Conclusion: The antioxidative effect of Neumentix was confirmed. This is the first report to demonstrate the antioxidative effect of Neumentix on strokes.",

keywords = "Infarction volume, Mint, ROS, Rosmarinic acid",

author = "Yuki Taira and Toru Yamashita and Yuting Bian and Jingwei Shang and Namiko Matsumoto and Ryo Sasaki and Koh Tadokoro and Emi Nomura and Keiichiro Tsunoda and Yosio Omote and Mami Takemoto and Nozomi Hishikawa and Yasuyuki Ohta and Koji Abe",

note = "Funding Information: Grant support: This work was partly supported by a Grant-in-Aid for Scientific Research (B) 17H04196, (C) 17K10827 and Challenging Research 15K15527, and by Grants-in-Aid from the Research Committees (Mizusawa H, Nishizawa M, Sasaki H, and Aoki M) from the Ministry of Health, Labour and Welfare of Japan. Funding Information: This work was partly supported by a Grant-in-Aid for Scientific Research (B) 17H04196, (C) 17K10827 and Challenging Research 15K15527, and by Grants-in-Aid from the Research Committees (Mizusawa H, Nishizawa M, Sasaki H, and Aoki M) from the Ministry of Health, Labour and Welfareof Japan. We are grateful to Dr. Maruyama of the Department of Preventive Dentistry at Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences for his technical assistance with the OXY-adsorbent and d-ROMs tests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = aug,

doi = "10.1016/j.jstrokecerebrovasdis.2020.104818",

language = "English",

volume = "29",

journal = "Journal of Stroke and Cerebrovascular Diseases",

issn = "1052-3057",

publisher = "W.B. Saunders Ltd",

number = "8",

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TY - JOUR

T1 - Antioxidative effects of a novel dietary supplement Neumentix in a mouse stroke model

AU - Taira, Yuki

AU - Yamashita, Toru

AU - Bian, Yuting

AU - Shang, Jingwei

AU - Matsumoto, Namiko

AU - Sasaki, Ryo

AU - Tadokoro, Koh

AU - Nomura, Emi

AU - Tsunoda, Keiichiro

AU - Omote, Yosio

AU - Takemoto, Mami

AU - Hishikawa, Nozomi

AU - Ohta, Yasuyuki

AU - Abe, Koji

N1 - Funding Information: Grant support: This work was partly supported by a Grant-in-Aid for Scientific Research (B) 17H04196, (C) 17K10827 and Challenging Research 15K15527, and by Grants-in-Aid from the Research Committees (Mizusawa H, Nishizawa M, Sasaki H, and Aoki M) from the Ministry of Health, Labour and Welfare of Japan. Funding Information: This work was partly supported by a Grant-in-Aid for Scientific Research (B) 17H04196, (C) 17K10827 and Challenging Research 15K15527, and by Grants-in-Aid from the Research Committees (Mizusawa H, Nishizawa M, Sasaki H, and Aoki M) from the Ministry of Health, Labour and Welfareof Japan. We are grateful to Dr. Maruyama of the Department of Preventive Dentistry at Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences for his technical assistance with the OXY-adsorbent and d-ROMs tests. Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/8

Y1 - 2020/8

N2 - Background: During an acute stroke, reactive oxygen species are overproduced and the endogenous antioxidative defense systems are disrupted. Therefore, antioxidative therapy can be a promising scheme to reduce the severity of stroke. Neumentix is a novel antioxidative supplement produced from a patented mint line and contains a high content of rosmarinic acid (RA). Although Neumentix has proven diverse efficacy and safety in clinical trials, its effect on strokes is unclear. Methods: Mice that were treated with Neumentix or vehicle for 14 days underwent transient middle cerebral artery occlusion (tMCAO) for 60 min. Mice were sacrificed 5 days after tMCAO. Results: Neumentix preserved body weight after tMCAO, showed a high antioxidative effect in serum, and reduced infarction volume compared to the vehicle. The expression of 4-hydroxy-2-nonenal, Nε-(carboxymethyl) lysine, and 8-hydroxy-2′-deoxyguanosine was reduced in Neumentix-treated mice. Conclusion: The antioxidative effect of Neumentix was confirmed. This is the first report to demonstrate the antioxidative effect of Neumentix on strokes.

AB - Background: During an acute stroke, reactive oxygen species are overproduced and the endogenous antioxidative defense systems are disrupted. Therefore, antioxidative therapy can be a promising scheme to reduce the severity of stroke. Neumentix is a novel antioxidative supplement produced from a patented mint line and contains a high content of rosmarinic acid (RA). Although Neumentix has proven diverse efficacy and safety in clinical trials, its effect on strokes is unclear. Methods: Mice that were treated with Neumentix or vehicle for 14 days underwent transient middle cerebral artery occlusion (tMCAO) for 60 min. Mice were sacrificed 5 days after tMCAO. Results: Neumentix preserved body weight after tMCAO, showed a high antioxidative effect in serum, and reduced infarction volume compared to the vehicle. The expression of 4-hydroxy-2-nonenal, Nε-(carboxymethyl) lysine, and 8-hydroxy-2′-deoxyguanosine was reduced in Neumentix-treated mice. Conclusion: The antioxidative effect of Neumentix was confirmed. This is the first report to demonstrate the antioxidative effect of Neumentix on strokes.

KW - Infarction volume

KW - Mint

KW - ROS

KW - Rosmarinic acid

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Antioxidative effects of a novel dietary supplement Neumentix in a mouse stroke model

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