TY - JOUR
T1 - Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli
AU - Kitamatsu, Mizuki
AU - Kurami, Shunsuke
AU - Ohtsuki, Takashi
AU - Sisido, Masahiko
N1 - Funding Information:
This study was supported by a Grand-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan (No. 19750143 ), Okayama Foundation for Science and Technology and The Kurata Memorial Hitachi Science and Technology Foundation .
PY - 2011/1/1
Y1 - 2011/1/1
N2 - To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.
AB - To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.
KW - Antisense effect
KW - Cell-penetrating peptide
KW - Peptide nucleic acid
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U2 - 10.1016/j.bmcl.2010.11.034
DO - 10.1016/j.bmcl.2010.11.034
M3 - Article
C2 - 21112781
AN - SCOPUS:78650515223
SN - 0960-894X
VL - 21
SP - 225
EP - 227
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 1
ER -