Apoptosis of tubular epithelial cells in rats with chronic renal failure

Hirofumi Makino; Hitoshi Sugiyama; Naoki Kashihara; Takashi Sekikawa; Tsuneto Onbe; Takanobu Nakajima; Yasushi Yamasaki; Zensuke Ota

doi:10.1007/BF02347965

Apoptosis of tubular epithelial cells in rats with chronic renal failure

Hirofumi Makino, Hitoshi Sugiyama, Naoki Kashihara, Takashi Sekikawa, Tsuneto Onbe, Takanobu Nakajima, Yasushi Yamasaki, Zensuke Ota

Research output: Contribution to journal › Article › peer-review

Abstract

Tubular injury leading to tubular atrophy and tubular loss is one of the characteristic features of chronic renal failure. To reveal the mechanism of tubular atrophy in chronic renal failure, the involvement of apoptosis was studied by in situ nick end labeling of biotinylated deoxyuridine by terminal deoxy nucleotidyl transferase (TUNEL) as well as by electron microscopy. TUNEL-positive cells and heterophagosomes containing apoptotic body-like structures having nuclear fragments with condensed chromatin were observed in the renal tubular epithelium 8 weeks after nephrectomy. It has become apparent from our study that atrophy of the tubules in chronic renal failure results, to some extent, from cell deletion by apoptosis.

Original language	English
Pages (from-to)	210-212
Number of pages	3
Journal	Medical Electron Microscopy
Volume	28
Issue number	3-4
DOIs	https://doi.org/10.1007/BF02347965
Publication status	Published - Dec 1 1995

Keywords

Apoptosis
Chronic renal failure
DNA fragmentation
Kidney
Renal tubules

ASJC Scopus subject areas

Anatomy

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10.1007/BF02347965

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title = "Apoptosis of tubular epithelial cells in rats with chronic renal failure",

abstract = "Tubular injury leading to tubular atrophy and tubular loss is one of the characteristic features of chronic renal failure. To reveal the mechanism of tubular atrophy in chronic renal failure, the involvement of apoptosis was studied by in situ nick end labeling of biotinylated deoxyuridine by terminal deoxy nucleotidyl transferase (TUNEL) as well as by electron microscopy. TUNEL-positive cells and heterophagosomes containing apoptotic body-like structures having nuclear fragments with condensed chromatin were observed in the renal tubular epithelium 8 weeks after nephrectomy. It has become apparent from our study that atrophy of the tubules in chronic renal failure results, to some extent, from cell deletion by apoptosis.",

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AU - Makino, Hirofumi

AU - Sugiyama, Hitoshi

AU - Kashihara, Naoki

AU - Sekikawa, Takashi

AU - Onbe, Tsuneto

AU - Nakajima, Takanobu

AU - Yamasaki, Yasushi

AU - Ota, Zensuke

PY - 1995/12/1

Y1 - 1995/12/1

N2 - Tubular injury leading to tubular atrophy and tubular loss is one of the characteristic features of chronic renal failure. To reveal the mechanism of tubular atrophy in chronic renal failure, the involvement of apoptosis was studied by in situ nick end labeling of biotinylated deoxyuridine by terminal deoxy nucleotidyl transferase (TUNEL) as well as by electron microscopy. TUNEL-positive cells and heterophagosomes containing apoptotic body-like structures having nuclear fragments with condensed chromatin were observed in the renal tubular epithelium 8 weeks after nephrectomy. It has become apparent from our study that atrophy of the tubules in chronic renal failure results, to some extent, from cell deletion by apoptosis.

AB - Tubular injury leading to tubular atrophy and tubular loss is one of the characteristic features of chronic renal failure. To reveal the mechanism of tubular atrophy in chronic renal failure, the involvement of apoptosis was studied by in situ nick end labeling of biotinylated deoxyuridine by terminal deoxy nucleotidyl transferase (TUNEL) as well as by electron microscopy. TUNEL-positive cells and heterophagosomes containing apoptotic body-like structures having nuclear fragments with condensed chromatin were observed in the renal tubular epithelium 8 weeks after nephrectomy. It has become apparent from our study that atrophy of the tubules in chronic renal failure results, to some extent, from cell deletion by apoptosis.

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KW - Chronic renal failure

KW - DNA fragmentation

KW - Kidney

KW - Renal tubules

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Apoptosis of tubular epithelial cells in rats with chronic renal failure

Abstract

Keywords

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