Apoptosis regulated by growth factors in multicellular spheroids of trabecular cells

Toshihiko Matsuo, Nobuhiko Matsuo

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6 Citations (Scopus)


Bovine and porcine trabecular cells were incubated up to one month in a polystyrene dish nonadherent environment for suspension culture, and their morphological changes were examined by light and electron microscopy. The trabecular cells formed multicellular spheroids during the first day. Apoptosis occurred selectively and continuously in the spheroids, which had initially been filled with cells. After one month, the final structure of the spheroids had become a meshwork of cells separated by large extracellular spaces. Epidermal and basic fibroblast growth factors prevented apoptosis of the trabecular cells in the spheroids which remained filled with cells for the entire observation period. The trabecular multicellular spheroids may represent the in vivo process of trabecular meshwork formation and can be used as a system for identifying regulatory factors involved in this process.

Original languageEnglish
Pages (from-to)356-366
Number of pages11
JournalJapanese Journal of Ophthalmology
Issue number3
Publication statusPublished - Nov 30 1996


  • Apoptosis
  • Basic fibroblast growth factor
  • Epidermal growth factor
  • Multicellular spheroids
  • Trabecular cells

ASJC Scopus subject areas

  • Ophthalmology


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