Aqueous extracts of Rhizopus oryzae induced nitric oxide production in rat hepatocyte cell line RLN-10

Takehito Suzuki; Mayuko Uchida; Yuji Takeda; Chiemi Mori; Atsushi Onuki; Yoko Miyazaki; Ken Onda; Setsuo Ushikoshi; Kotaro Shitori; Kazuaki Tanaka; Hidetoshi Morita; Tatsuya Takizawa

doi:10.1271/bbb.120951

Aqueous extracts of Rhizopus oryzae induced nitric oxide production in rat hepatocyte cell line RLN-10

Takehito Suzuki, Mayuko Uchida, Yuji Takeda, Chiemi Mori, Atsushi Onuki, Yoko Miyazaki, Ken Onda, Setsuo Ushikoshi, Kotaro Shitori, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

Research output: Contribution to journal › Article › peer-review

Abstract

Aqueous extracts of Rhizopus oryzae (Aq-ROU) have a broad range of physiological activity. Here we identified a new physiological effect of Aq-ROU in rat hepatocyte cell line RLN-10. Aq-ROU induced the accumulation of nitrite, a stable metabolite nitric oxide (NO), in cell culture medium and induced potent diaminofluorescein-FM diacetate staining in the cells. Real-time reverse transcriptase (RT)-PCR analysis showed marked inducible NO synthase gene expression. Additionally, markedly enhanced expression of p22^phox and temporally increased expression of NADPH oxidase1 indicated that superoxide was produced. Nuclear translocation of nuclear factor-kappa (NF-κ) B p65 increased remarkably following Aq-ROU and following lipopolysaccharide treatment, a potent activator of NF-κB. Ammonium pyrrolidine-1- carbodithioate, an inhibitor of NF-κB, inhibited NO production following Aq-ROU treatment. Our data indicate that Aq-ROU induces NO production and potentially the production of superoxide, which may contribute to the broad range of physiological effects observed for Aq-ROU ingested by animals.

Original language	English
Pages (from-to)	1384-1389
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	77
Issue number	7
DOIs	https://doi.org/10.1271/bbb.120951
Publication status	Published - 2013
Externally published	Yes

Keywords

Ammonium pyrrolidine-1-carbodithioate (APDC)
Hepatocyte cell line
Nitric oxide (NO)
Nuclear factor-κB
Rhizopus oryzae

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.120951

Cite this

@article{da0ac87c25e2487889399c3b7a01075d,

title = "Aqueous extracts of Rhizopus oryzae induced nitric oxide production in rat hepatocyte cell line RLN-10",

abstract = "Aqueous extracts of Rhizopus oryzae (Aq-ROU) have a broad range of physiological activity. Here we identified a new physiological effect of Aq-ROU in rat hepatocyte cell line RLN-10. Aq-ROU induced the accumulation of nitrite, a stable metabolite nitric oxide (NO), in cell culture medium and induced potent diaminofluorescein-FM diacetate staining in the cells. Real-time reverse transcriptase (RT)-PCR analysis showed marked inducible NO synthase gene expression. Additionally, markedly enhanced expression of p22phox and temporally increased expression of NADPH oxidase1 indicated that superoxide was produced. Nuclear translocation of nuclear factor-kappa (NF-κ) B p65 increased remarkably following Aq-ROU and following lipopolysaccharide treatment, a potent activator of NF-κB. Ammonium pyrrolidine-1- carbodithioate, an inhibitor of NF-κB, inhibited NO production following Aq-ROU treatment. Our data indicate that Aq-ROU induces NO production and potentially the production of superoxide, which may contribute to the broad range of physiological effects observed for Aq-ROU ingested by animals.",

keywords = "Ammonium pyrrolidine-1-carbodithioate (APDC), Hepatocyte cell line, Nitric oxide (NO), Nuclear factor-κB, Rhizopus oryzae",

author = "Takehito Suzuki and Mayuko Uchida and Yuji Takeda and Chiemi Mori and Atsushi Onuki and Yoko Miyazaki and Ken Onda and Setsuo Ushikoshi and Kotaro Shitori and Kazuaki Tanaka and Hidetoshi Morita and Tatsuya Takizawa",

note = "Funding Information: We thank Mr. Kazuaki Ishii for preparing the manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research (no. 24780259) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by a Grant-in-Aid for Matching Fund Subsidies for Private Universities from the Promotion and Mutual Aid Corporation for Private Schools of Japan.",

year = "2013",

doi = "10.1271/bbb.120951",

language = "English",

volume = "77",

pages = "1384--1389",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "7",

}

TY - JOUR

T1 - Aqueous extracts of Rhizopus oryzae induced nitric oxide production in rat hepatocyte cell line RLN-10

AU - Suzuki, Takehito

AU - Uchida, Mayuko

AU - Takeda, Yuji

AU - Mori, Chiemi

AU - Onuki, Atsushi

AU - Miyazaki, Yoko

AU - Onda, Ken

AU - Ushikoshi, Setsuo

AU - Shitori, Kotaro

AU - Tanaka, Kazuaki

AU - Morita, Hidetoshi

AU - Takizawa, Tatsuya

N1 - Funding Information: We thank Mr. Kazuaki Ishii for preparing the manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research (no. 24780259) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by a Grant-in-Aid for Matching Fund Subsidies for Private Universities from the Promotion and Mutual Aid Corporation for Private Schools of Japan.

PY - 2013

Y1 - 2013

N2 - Aqueous extracts of Rhizopus oryzae (Aq-ROU) have a broad range of physiological activity. Here we identified a new physiological effect of Aq-ROU in rat hepatocyte cell line RLN-10. Aq-ROU induced the accumulation of nitrite, a stable metabolite nitric oxide (NO), in cell culture medium and induced potent diaminofluorescein-FM diacetate staining in the cells. Real-time reverse transcriptase (RT)-PCR analysis showed marked inducible NO synthase gene expression. Additionally, markedly enhanced expression of p22phox and temporally increased expression of NADPH oxidase1 indicated that superoxide was produced. Nuclear translocation of nuclear factor-kappa (NF-κ) B p65 increased remarkably following Aq-ROU and following lipopolysaccharide treatment, a potent activator of NF-κB. Ammonium pyrrolidine-1- carbodithioate, an inhibitor of NF-κB, inhibited NO production following Aq-ROU treatment. Our data indicate that Aq-ROU induces NO production and potentially the production of superoxide, which may contribute to the broad range of physiological effects observed for Aq-ROU ingested by animals.

AB - Aqueous extracts of Rhizopus oryzae (Aq-ROU) have a broad range of physiological activity. Here we identified a new physiological effect of Aq-ROU in rat hepatocyte cell line RLN-10. Aq-ROU induced the accumulation of nitrite, a stable metabolite nitric oxide (NO), in cell culture medium and induced potent diaminofluorescein-FM diacetate staining in the cells. Real-time reverse transcriptase (RT)-PCR analysis showed marked inducible NO synthase gene expression. Additionally, markedly enhanced expression of p22phox and temporally increased expression of NADPH oxidase1 indicated that superoxide was produced. Nuclear translocation of nuclear factor-kappa (NF-κ) B p65 increased remarkably following Aq-ROU and following lipopolysaccharide treatment, a potent activator of NF-κB. Ammonium pyrrolidine-1- carbodithioate, an inhibitor of NF-κB, inhibited NO production following Aq-ROU treatment. Our data indicate that Aq-ROU induces NO production and potentially the production of superoxide, which may contribute to the broad range of physiological effects observed for Aq-ROU ingested by animals.

KW - Ammonium pyrrolidine-1-carbodithioate (APDC)

KW - Hepatocyte cell line

KW - Nitric oxide (NO)

KW - Nuclear factor-κB

KW - Rhizopus oryzae

UR - http://www.scopus.com/inward/record.url?scp=84882772475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84882772475&partnerID=8YFLogxK

U2 - 10.1271/bbb.120951

DO - 10.1271/bbb.120951

M3 - Article

AN - SCOPUS:84882772475

SN - 0916-8451

VL - 77

SP - 1384

EP - 1389

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 7

ER -

Aqueous extracts of Rhizopus oryzae induced nitric oxide production in rat hepatocyte cell line RLN-10

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this