Are glucocorticoids auto- and/or paracrine factors in early bovine embryo development and implantation?

Marta J. Siemieniuch; Magdalena Majewska; Masashi Takahashi; Miki Sakatani; Karolina Łukasik; Kiyoshi Okuda; Dariusz J. Skarzynski

doi:10.1016/S1642-431X(12)60045-X

Are glucocorticoids auto- and/or paracrine factors in early bovine embryo development and implantation?

Marta J. Siemieniuch, Magdalena Majewska, Masashi Takahashi, Miki Sakatani, Karolina Łukasik, Kiyoshi Okuda, Dariusz J. Skarzynski

Faculty of Agriculture

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We determined the transcript content of three genes involved in the metabolism of glucocorticoids (GC) in bovine in vitro fertilized embryos (2-blastomere stage until hatched blastocyst), trophoblast as well as the oviduct (Day 2-4 of the estrous cycle) and endometrium (Day 16 of the cycle and pregnancy). Since mRNA expression of the glucocorticoid receptor and two enzymes responsible for GC production (11β-HSD1 and 2) was demonstrated in the embryos in all pre-implantation stages as well as in the endometrium and oviduct, it is suggested that GC may serve as auto-/paracrine factors in the development of bovine pre-implantation embryos.

Original language	English
Pages (from-to)	249-256
Number of pages	8
Journal	Reproductive Biology
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/S1642-431X(12)60045-X
Publication status	Published - Nov 2010

Keywords

Cattle
Embryo
Endometrium
Glucocorticoids
Oviduct
Trophoblast

ASJC Scopus subject areas

Animal Science and Zoology
Endocrinology
Developmental Biology

Access to Document

10.1016/S1642-431X(12)60045-X

Cite this

@article{09bf5419f9bd45909719fcaf3d002dfc,

title = "Are glucocorticoids auto- and/or paracrine factors in early bovine embryo development and implantation?",

abstract = "We determined the transcript content of three genes involved in the metabolism of glucocorticoids (GC) in bovine in vitro fertilized embryos (2-blastomere stage until hatched blastocyst), trophoblast as well as the oviduct (Day 2-4 of the estrous cycle) and endometrium (Day 16 of the cycle and pregnancy). Since mRNA expression of the glucocorticoid receptor and two enzymes responsible for GC production (11β-HSD1 and 2) was demonstrated in the embryos in all pre-implantation stages as well as in the endometrium and oviduct, it is suggested that GC may serve as auto-/paracrine factors in the development of bovine pre-implantation embryos.",

keywords = "Cattle, Embryo, Endometrium, Glucocorticoids, Oviduct, Trophoblast",

author = "Siemieniuch, {Marta J.} and Magdalena Majewska and Masashi Takahashi and Miki Sakatani and Karolina {\L}ukasik and Kiyoshi Okuda and Skarzynski, {Dariusz J.}",

note = "Funding Information: We thank Natsuko Matsumoto, Chiaki Sato, Michie Teramoto and Etsuko Kawano for their experimental assistance. We also thank Kumamoto Meat Trading Center Ltd. and Kumamoto Prefecture Meat Inspection Office for helping us to collect bovine ovaries. This work was supported by grants–in–aid of Polish Ministry of Sciences and Higher Education (No. 308327933), the Ministry of Agriculture, Forestry and Fisheries of Japan (Development of stable production technology of cloned animals by somatic cell nuclear transfer).",

year = "2010",

month = nov,

doi = "10.1016/S1642-431X(12)60045-X",

language = "English",

volume = "10",

pages = "249--256",

journal = "Reproductive biology",

issn = "1642-431X",