Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription

Katsuhiko Amano, Kenji Hata, Shuji Muramatsu, Makoto Wakabayashi, Yoko Takigawa, Koichiro Ono, Masako Nakanishi, Rikako Takashima, Mikihiko Kogo, Akio Matsuda, Riko Nishimura, Toshiyuki Yoneda

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


SRY-box-containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further understand the molecular mechanisms by which Sox9 influences transcriptional events during chondrocyte differentiation, we attempted to identify transcriptional partners of Sox9 and to examine their roles in chondrocyte differentiation. We isolated AT-rich interactive domain-containing protein 5a (Arid5a; also known as Mrf1) as an activator of the Col2a1 gene promoter from an ATDC5 cDNA library. Arid5a was highly expressed in cartilage and induced during chondrocyte differentiation. Furthermore, Arid5a physically interacted with Sox9 in nuclei and up-regulated the chondrocyte-specific action of Sox9. Overexpression of Arid5a stimulated chondrocyte differentiation in vitro and in an organ culture system. In contrast, Arid5a knockdown inhibited Col2a1 expression in chondrocytes. In addition, Arid5a binds directly to the promoter region of the Col2a1 gene and stimulates acetylation of histone 3 in the region. Our results suggest that Arid5a may directly interact with Sox9 and thereby enhance its chondrocyte-specific action.

Original languageEnglish
Pages (from-to)1300-1311
Number of pages12
JournalMolecular Biology of the Cell
Issue number8
Publication statusPublished - Apr 15 2011
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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