Artificial incubation of Japanese crayfish (Cambaroides japonicus) eggs by using a simple, easy method with a microplate

Kazuyoshi Nakata, Hajime Matsubara, Seiji Goshima

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


We developed a simple, easy method with a microplate to artificially incubate Japanese crayfish (Cambaroides japonicus) eggs for their cultivation. We prepared 6-, 12-, 24-, 48-, and 96-well microplates containing sterile water heated to 5, 10, 15, and 20°C. Fourteen experimental groups for each water temperature were prepared for each of different water volumes (0.125-10 ml) in each well. One embryonic egg was placed in each well. Experiments were also conducted with water collected from the lake where the eggs were harvested from and held at 15°C. In the microplates with sterile water, high proportions of eggs hatched (60-100%) at 15°C in all volumes of water, although the proportions of hatching were low (0-20%) at 5, 10, and 20°C. All eggs died in the experiments that used lake water. We conclude that the 96-well would be the most effective size to hatch crayfish eggs in, because of its convenience. This method using a microplate is simpler and easier compared with methods of previous studies to artificially incubate crayfish eggs, and therefore it might be useful to incubate eggs of other freshwater crayfish species.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
Issue number1-4
Publication statusPublished - Feb 16 2004
Externally publishedYes


  • Artificial incubation
  • Cambaroides japonicus
  • Cultivation
  • Egg
  • Japanese crayfish

ASJC Scopus subject areas

  • Aquatic Science


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