Assessment of intraoral mucosal pain induced by the application of capsaicin

Ryuji Terayama; Kotaro Maruhama; Hiroki Tsuchiya; Masahide Mizutani; Seiji Iida; Tomosada Sugimoto

doi:10.1016/j.archoralbio.2014.08.008

Assessment of intraoral mucosal pain induced by the application of capsaicin

Ryuji Terayama, Kotaro Maruhama, Hiroki Tsuchiya, Masahide Mizutani, Seiji Iida, Tomosada Sugimoto

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Objective To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN). Design Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN. Results Larger doses of intraoral capsaicin (1, 10 and 100 μg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 μg doses of capsaicin were significantly greater than that by the 1 μg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin. Conclusions The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury.

Original language	English
Pages (from-to)	1334-1341
Number of pages	8
Journal	Archives of Oral Biology
Volume	59
Issue number	12
DOIs	https://doi.org/10.1016/j.archoralbio.2014.08.008
Publication status	Published - Dec 2014

Keywords

Behaviour
Capsaicin
Immunohistochemistry
Medullary dorsal horn
Nerve injury
c-Fos

ASJC Scopus subject areas

Otorhinolaryngology
Dentistry(all)
Cell Biology

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10.1016/j.archoralbio.2014.08.008

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title = "Assessment of intraoral mucosal pain induced by the application of capsaicin",

abstract = "Objective To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN). Design Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN. Results Larger doses of intraoral capsaicin (1, 10 and 100 μg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 μg doses of capsaicin were significantly greater than that by the 1 μg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin. Conclusions The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury.",

keywords = "Behaviour, Capsaicin, Immunohistochemistry, Medullary dorsal horn, Nerve injury, c-Fos",

author = "Ryuji Terayama and Kotaro Maruhama and Hiroki Tsuchiya and Masahide Mizutani and Seiji Iida and Tomosada Sugimoto",

note = "Funding Information: This study was supported by a Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science ( 24592764 ). All authors report no financial relationship related to any product involved in this study. ",

year = "2014",

month = dec,

doi = "10.1016/j.archoralbio.2014.08.008",

language = "English",

volume = "59",

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journal = "Archives of Oral Biology",

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T1 - Assessment of intraoral mucosal pain induced by the application of capsaicin

AU - Terayama, Ryuji

AU - Maruhama, Kotaro

AU - Tsuchiya, Hiroki

AU - Mizutani, Masahide

AU - Iida, Seiji

AU - Sugimoto, Tomosada

N1 - Funding Information: This study was supported by a Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science ( 24592764 ). All authors report no financial relationship related to any product involved in this study.

PY - 2014/12

Y1 - 2014/12

N2 - Objective To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN). Design Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN. Results Larger doses of intraoral capsaicin (1, 10 and 100 μg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 μg doses of capsaicin were significantly greater than that by the 1 μg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin. Conclusions The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury.

AB - Objective To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN). Design Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN. Results Larger doses of intraoral capsaicin (1, 10 and 100 μg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 μg doses of capsaicin were significantly greater than that by the 1 μg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin. Conclusions The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury.

KW - Behaviour

KW - Capsaicin

KW - Immunohistochemistry

KW - Medullary dorsal horn

KW - Nerve injury

KW - c-Fos

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Assessment of intraoral mucosal pain induced by the application of capsaicin

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