@article{01cceb5314074ccdb5ada2a740ad157b,
title = "Association of N-ethylmaleimide-sensitive factor with synaptic vesicles",
abstract = "N-Ethylmaleimide-sensitive factor (NSF) mediates docking and/or fusion of transport vesicles in the multi-pathways of vesicular transport. NSF was highly expressed in brain and adrenal gland. Immunostaining of cerebellum with an anti-NSF monoclonal antibody showed that NSF is predominantly localized in the molecular layers and the glomeruli of the granule cell layers. This distribution coincided well with that of synaptophysin, a marker protein of synaptic vesicles. Purification and immunoprecipitation revealed that NSF is associated with brain synaptic vesicles. The present results suggest that NSF is associated with synaptic vesicles without Ca2+ influx.",
keywords = "Neurotransmitter, Synaptic vesicle, Vesicular transport",
author = "Hong, {Rong Mao} and Hiroyuki Mori and Toshio Fukui and Yoshinori Moriyama and Masamitsu Futai and Akitsugu Yamamoto and Yutaka Tashiro and Mitsuo Tagaya",
note = "Funding Information: Acknowledgements: We thank Dr. Shoji Mizushima for encouragement of this study. We appreciate Mr. Shuich Toyonaga for his technical assistance.T his work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, Nissan Science Foundation, and Takeda Science Foundation. Funding Information: from the SNARE complex, which allows the association of NSF with the SNARE complex via SNAP. Subsequent hydrolysis of ATP by NSF causes the disassembly of the 20 S complex and triggers the fusion of synaptic vesicles with the plasma membrane [lo]. This hypothesis is quite an attractive explanation of the mechanism of Ca2+-regulated neurotransmitter secretion, but remains to be elucidated. It is known that NSF is present in membranes as well as in cytosol [2]. NSF may form the 20 S complex on synaptic vesicles without Ca” influx. This idea was supported by the present observation that NSF is associated with synaptic vesicles.",
year = "1994",
month = aug,
day = "22",
doi = "10.1016/0014-5793(94)00778-0",
language = "English",
volume = "350",
pages = "253--257",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2-3",
}