Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis

Hiroyuki Nakamura, Fumiko Higashikawa, Kiyoshi Miyagawa, Yoshitaka Nobukuni, Tomohiko Endo, Toru Imai, Kotaro Ozasa, Yutaka Motohashi, Ichiyo Matsuzaki, Shinichiro Sasahara, Kotaro Hatta, Keiki Ogino, Akira Eboshida

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10 Citations (Scopus)


Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

Original languageEnglish
Pages (from-to)40-43
Number of pages4
JournalInternational archives of allergy and immunology
Issue number1
Publication statusPublished - 2004
Externally publishedYes


  • Cedar pollinosis
  • Eosinophil peroxidase
  • Polymorphisms
  • Transmission disequilibrium test

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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