Astrocytes express type VIII collagen during the repair process of brain cold injury

Shuichi Hirano, Tomoko Yonezawa, Haruko Hasegawa, Shunji Hattori, Nicholas S. Greenhill, Paul F. Davis, E. Helene Sage, Yoshifumi Ninomiya

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


We recognized for the first time upregulation of type VIII collagen gene expression during the repair process in the mouse brain cold injury model. Immunohistochemical staining showed that type VIII collagen expression was around the necrotic region, where reactive astrocytes are frequently observed. Cultured astrocytes demonstrated a high expression of type VIII collagen genes. TGF-β1 enhanced the expression of both α1(VIII) and α2(VIII) genes by astrocytes in culture. Further, we tested selected biological activities of type VIII collagen, compared with those of type I, IV, and V collagens and fibronectin. Astrocytes adhered to type VIII collagen via receptors requiring metal ions. Astrocyte migration on type VIII collagen was more stimulated than that observed on the other ECM molecules. These data indicate that type VIII collagen plays an important role in glial scar formation during the repair process by astrocytes.

Original languageEnglish
Pages (from-to)437-443
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Apr 30 2004


  • Adhesion
  • Astrocyte
  • Brain
  • Cold injury model
  • Extracellular matrix
  • Glial fibrillary acidic protein
  • Glial scar
  • Migration
  • Transforming growth factor-β1
  • Type VIII collagen

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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