TY - JOUR
T1 - Asymmetric reduction of a variety of ketones with a recombinant carbonyl reductase
T2 - Identification of the gene encoding a versatile biocatalyst
AU - Ema, Tadashi
AU - Yagasaki, Hideo
AU - Okita, Nobuyasu
AU - Nishikawa, Kumiko
AU - Korenaga, Toshinobu
AU - Sakai, Takashi
N1 - Funding Information:
We thank Dr. Hiroaki Yamamoto (Daicel Chemical Industries) and Prof. Tetsuo Toraya (Okayama University) for the determination of the internal amino-acid sequences of SCR and useful advice on gene expression, respectively. This work was supported by a Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science and by a grant from Venture Business Laboratory of Okayama University. We are grateful to the SC-NMR Laboratory of Okayama University for the measurement of NMR spectra.
PY - 2005/3/21
Y1 - 2005/3/21
N2 - The gene encoding a versatile biocatalyst that shows high enantioselectivity for a variety of ketones, SCR (Saccharomyces cerevisiae carbonyl reductase), has been identified, cloned, and expressed in E. coli. Recombinant E. coli co-producing SCR and GDH (glucose dehydrogenase) is an easy-to-use, synthetically useful biocatalyst, and 8 out of the 16 alcohols obtained had enantiomeric purities of >98% ee.
AB - The gene encoding a versatile biocatalyst that shows high enantioselectivity for a variety of ketones, SCR (Saccharomyces cerevisiae carbonyl reductase), has been identified, cloned, and expressed in E. coli. Recombinant E. coli co-producing SCR and GDH (glucose dehydrogenase) is an easy-to-use, synthetically useful biocatalyst, and 8 out of the 16 alcohols obtained had enantiomeric purities of >98% ee.
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U2 - 10.1016/j.tetasy.2005.02.004
DO - 10.1016/j.tetasy.2005.02.004
M3 - Article
AN - SCOPUS:14944383719
SN - 0957-4166
VL - 16
SP - 1075
EP - 1078
JO - Tetrahedron Asymmetry
JF - Tetrahedron Asymmetry
IS - 6
ER -