Asymmetric reduction of ethyl 2-methyl 3-oxobutanoate by chlorella

Takayuki Kuramoto, Kenzo Iwamoto, Minoru Izumi, Mitsunori Kirihata, Fumiki Yoshizako

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Chlorella pyrenoidosa Chick reduced ethyl 2-methyl 3-oxobutanoate to the corresponding alcohols with the diastereomer (anti/syn) ratio of 53/47. The enantiomer excesses of anti-(2S, 3S)- and syn-(2S, 3R)-hydroxy esters were 89 and >99ee% respectively. C. vulgaris and C. regularis afforded predominantly the syn-isomer, contrary to C. pyrenoidosa. The differences in the activity of reducing ethyl 2-methyl 3-oxobutanoate were observed among three strains of Chlorella. Addition of 2% metal salts slightly increased the chemical yield of the hydroxy ester.

Original languageEnglish
Pages (from-to)598-601
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - Jan 1 1999
Externally publishedYes


  • (2S, 3R)-ethyl 2-methyl 3-hydroxybutanoate
  • (2S, 3S)-ethyl 2-methyl 3-hydroxybutanoate
  • Asymmetric reduction
  • Chlorella
  • Ethyl 2-methyl 3-oxobutanoate

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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