@article{521b4e99d2684f5393a2c3fab51a2ea7,
title = "Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG",
abstract = "Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.",
keywords = "BCG, Mycobacterium, ThyX, thymidylate synthase",
author = "Yuki Arimura and Yusuke Minato and Takayuki Wada and Masaaki Nakayama and Ayako Ryumon and Nao Hirata and Chie Nakajima and Yasuhiko Suzuki and Manabu Ato and Kazuo Kobayashi and Naoko Ohara and Seiji Iida and Naoya Ohara",
note = "Funding Information: The authors thank Professor Koji Nakayama and Mayuko Okabe for helpful discussions. JSPS KAKENHI Grant Number 16K15277 (for Naoya Ohara). Agency for Medical Research and Development (AMED) Grant Numbers JP20fk0108129 (for Manabu Ato), JP21fk0108090 (for Naoya Ohara), and JP21fk0108607 (for Yusuke Minato). The grant from Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) for the Joint Research Program of the Hokkaido University Research Center for Zoonosis Control (for Masaaki Nakayama and Naoya Ohara). Publisher Copyright: {\textcopyright} 2021 The Societies and John Wiley & Sons Australia, Ltd",
year = "2021",
doi = "10.1111/1348-0421.12944",
language = "English",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
}
