TY - GEN
T1 - Automated drug screening system for ion channel proteins
AU - Kawano, R.
AU - Tsuji, Y.
AU - Hirano, M.
AU - Osaki, T.
AU - Sasaki, H.
AU - Kamiya, K.
AU - Miki, N.
AU - Ide, T.
AU - Takeuchi, S.
PY - 2011
Y1 - 2011
N2 - This paper describes an automated drug screening system for Ca 2+-activated K+ channel proteins (BK channel). BK channels are pharmacological targets for the treatment of stroke. We have been developing multiple lipid bilayer membranes (BLMs) system for reconstituting the membrane proteins [1]. In this study, the osmotic pressure in the proteoliposomes was controlled to induce the vesicle fusion. As a result, we succeeded in simultaneous reconstitution of BK channels in the BLMs array using an injection robot (Fig. 1). Moreover, the channel inhibition behavior was examined using a K+ channel blocker. Since our method is applicable for multi-type of proteoliposomes, we believe that the system can be used as a high-throughput screening (HTS) system for membrane-protein-based drug discovery.
AB - This paper describes an automated drug screening system for Ca 2+-activated K+ channel proteins (BK channel). BK channels are pharmacological targets for the treatment of stroke. We have been developing multiple lipid bilayer membranes (BLMs) system for reconstituting the membrane proteins [1]. In this study, the osmotic pressure in the proteoliposomes was controlled to induce the vesicle fusion. As a result, we succeeded in simultaneous reconstitution of BK channels in the BLMs array using an injection robot (Fig. 1). Moreover, the channel inhibition behavior was examined using a K+ channel blocker. Since our method is applicable for multi-type of proteoliposomes, we believe that the system can be used as a high-throughput screening (HTS) system for membrane-protein-based drug discovery.
KW - BK channels
KW - High-throughput screenings
KW - Lipid bilayers
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M3 - Conference contribution
AN - SCOPUS:84883806034
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 76
EP - 78
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -