BDNF-secreting capsule exerts neuroprotective effects on epilepsy model of rats

Satoshi Kuramoto; Takao Yasuhara; Takashi Agari; Akihiko Kondo; Meng Jing; Yoichiro Kikuchi; Aiko Shinko; Takaaki Wakamori; Masahiro Kameda; Feifei Wang; Kyohei Kin; Satoru Edahiro; Yasuyuki Miyoshi; Isao Date

doi:10.1016/j.brainres.2010.10.054

BDNF-secreting capsule exerts neuroprotective effects on epilepsy model of rats

Satoshi Kuramoto, Takao Yasuhara, Takashi Agari, Akihiko Kondo, Meng Jing, Yoichiro Kikuchi, Aiko Shinko, Takaaki Wakamori, Masahiro Kameda, Feifei Wang, Kyohei Kin, Satoru Edahiro, Yasuyuki Miyoshi, Isao Date

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25 Citations (Scopus)

Abstract

Brain-derived neurotrophic factor (BDNF) is a well neurotrophic factor with neuroprotective potentials for various diseases in the central nervous system. However several previous studies demonstrated that BDNF might deteriorate symptoms for epilepsy model of animals by progression of abnormal neurogenesis. We hypothesized that continuous administration of BDNF at low dose might be more effective for epilepsy model of animals because high dose of BDNF was used in many studies. BDNF-secreting cells were genetically made and encapsulated for transplantation. Rats receiving BDNF capsule showed significant amelioration of seizure stage and reduction of the number of abnormal spikes at 7 days after kainic acid administration, compared to those of control group. The number of BrdU and BrdU/doublecortin positive cells in the hippocampus of BDNF group significantly increased, compared to that of control group. NeuN positive cells in the CA1 and CA3 of BDNF group were significantly preserved, compared to control group. In conclusion, low dose administration using encapsulated BDNF-secreting cells exerted neuroprotective effects with enhanced neurogenesis on epilepsy model of rats. These results might suggest the importance of the dose and administrative way of this neurotrophic factor to the epilepsy model of animals.

Original language	English
Pages (from-to)	281-289
Number of pages	9
Journal	Brain Research
Volume	1368
DOIs	https://doi.org/10.1016/j.brainres.2010.10.054
Publication status	Published - Jan 12 2011

Keywords

Brain-derived neurotrophic factor
Epilepsy
Ex vivo gene therapy
Kainic acid
Neurogenesis
Neuroprotection

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2010.10.054

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@article{57f51bea51dd43bc89caf315df6dd599,

title = "BDNF-secreting capsule exerts neuroprotective effects on epilepsy model of rats",

abstract = "Brain-derived neurotrophic factor (BDNF) is a well neurotrophic factor with neuroprotective potentials for various diseases in the central nervous system. However several previous studies demonstrated that BDNF might deteriorate symptoms for epilepsy model of animals by progression of abnormal neurogenesis. We hypothesized that continuous administration of BDNF at low dose might be more effective for epilepsy model of animals because high dose of BDNF was used in many studies. BDNF-secreting cells were genetically made and encapsulated for transplantation. Rats receiving BDNF capsule showed significant amelioration of seizure stage and reduction of the number of abnormal spikes at 7 days after kainic acid administration, compared to those of control group. The number of BrdU and BrdU/doublecortin positive cells in the hippocampus of BDNF group significantly increased, compared to that of control group. NeuN positive cells in the CA1 and CA3 of BDNF group were significantly preserved, compared to control group. In conclusion, low dose administration using encapsulated BDNF-secreting cells exerted neuroprotective effects with enhanced neurogenesis on epilepsy model of rats. These results might suggest the importance of the dose and administrative way of this neurotrophic factor to the epilepsy model of animals.",

keywords = "Brain-derived neurotrophic factor, Epilepsy, Ex vivo gene therapy, Kainic acid, Neurogenesis, Neuroprotection",

author = "Satoshi Kuramoto and Takao Yasuhara and Takashi Agari and Akihiko Kondo and Meng Jing and Yoichiro Kikuchi and Aiko Shinko and Takaaki Wakamori and Masahiro Kameda and Feifei Wang and Kyohei Kin and Satoru Edahiro and Yasuyuki Miyoshi and Isao Date",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology , JAPAN; and by a Research Grant from the Japan Epilepsy Research Foundation .",

year = "2011",

month = jan,

day = "12",

doi = "10.1016/j.brainres.2010.10.054",

language = "English",

volume = "1368",

pages = "281--289",

journal = "Brain Research",

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T1 - BDNF-secreting capsule exerts neuroprotective effects on epilepsy model of rats

AU - Kuramoto, Satoshi

AU - Yasuhara, Takao

AU - Agari, Takashi

AU - Kondo, Akihiko

AU - Jing, Meng

AU - Kikuchi, Yoichiro

AU - Shinko, Aiko

AU - Wakamori, Takaaki

AU - Kameda, Masahiro

AU - Wang, Feifei

AU - Kin, Kyohei

AU - Edahiro, Satoru

AU - Miyoshi, Yasuyuki

AU - Date, Isao

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology , JAPAN; and by a Research Grant from the Japan Epilepsy Research Foundation .

PY - 2011/1/12

Y1 - 2011/1/12

N2 - Brain-derived neurotrophic factor (BDNF) is a well neurotrophic factor with neuroprotective potentials for various diseases in the central nervous system. However several previous studies demonstrated that BDNF might deteriorate symptoms for epilepsy model of animals by progression of abnormal neurogenesis. We hypothesized that continuous administration of BDNF at low dose might be more effective for epilepsy model of animals because high dose of BDNF was used in many studies. BDNF-secreting cells were genetically made and encapsulated for transplantation. Rats receiving BDNF capsule showed significant amelioration of seizure stage and reduction of the number of abnormal spikes at 7 days after kainic acid administration, compared to those of control group. The number of BrdU and BrdU/doublecortin positive cells in the hippocampus of BDNF group significantly increased, compared to that of control group. NeuN positive cells in the CA1 and CA3 of BDNF group were significantly preserved, compared to control group. In conclusion, low dose administration using encapsulated BDNF-secreting cells exerted neuroprotective effects with enhanced neurogenesis on epilepsy model of rats. These results might suggest the importance of the dose and administrative way of this neurotrophic factor to the epilepsy model of animals.

AB - Brain-derived neurotrophic factor (BDNF) is a well neurotrophic factor with neuroprotective potentials for various diseases in the central nervous system. However several previous studies demonstrated that BDNF might deteriorate symptoms for epilepsy model of animals by progression of abnormal neurogenesis. We hypothesized that continuous administration of BDNF at low dose might be more effective for epilepsy model of animals because high dose of BDNF was used in many studies. BDNF-secreting cells were genetically made and encapsulated for transplantation. Rats receiving BDNF capsule showed significant amelioration of seizure stage and reduction of the number of abnormal spikes at 7 days after kainic acid administration, compared to those of control group. The number of BrdU and BrdU/doublecortin positive cells in the hippocampus of BDNF group significantly increased, compared to that of control group. NeuN positive cells in the CA1 and CA3 of BDNF group were significantly preserved, compared to control group. In conclusion, low dose administration using encapsulated BDNF-secreting cells exerted neuroprotective effects with enhanced neurogenesis on epilepsy model of rats. These results might suggest the importance of the dose and administrative way of this neurotrophic factor to the epilepsy model of animals.

KW - Brain-derived neurotrophic factor

KW - Epilepsy

KW - Ex vivo gene therapy

KW - Kainic acid

KW - Neurogenesis

KW - Neuroprotection

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JO - Brain Research

JF - Brain Research

ER -

BDNF-secreting capsule exerts neuroprotective effects on epilepsy model of rats

Abstract

Keywords

ASJC Scopus subject areas

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