TY - JOUR
T1 - BIG-2 Mediates Olfactory Axon Convergence to Target Glomeruli
AU - Kaneko-Goto, Tomomi
AU - Yoshihara, Sei ichi
AU - Miyazaki, Haruko
AU - Yoshihara, Yoshihiro
N1 - Funding Information:
We thank K. Mori for continuous support and encouragement; H. Sakano for gifts of anti-Kirrel2 antibody and H-MOR28-ires-tauECFP transgenic mice; H. Breer for a gift of anti-mOR256-17 antibody; F.L. Margolis for a gift of anti-OMP antibody; T. Kaneko for a gift of anti-VGluT2 antibody; Y.F. Sasaki for help in production of anti-BIG-2 antibody; S. Mitsui for help in production of anti-protocadherin-21 antibody; K. Kubota and M. Kawasaki for help in construction of BIG-2 targeting vector; Y. Furutani for help in production of recombinant proteins; BSI Research Resource Center (RRC) for help in generation of BIG-2-deficient mice; N. Miyasaka for comments on the manuscript; and members of the Yoshihara lab for valuable discussions. This work was supported in part by a Grant-in-Aid for Scientific Research (B) and a Grant-in-Aid for Scientific Research on Priority Area (Cellular Sensor) to Y.Y. from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2008/3/27
Y1 - 2008/3/27
N2 - Olfactory sensory neurons expressing a given odorant receptor converge axons onto a few topographically fixed glomeruli in the olfactory bulb, leading to establishment of the odor map. Here, we report that BIG-2/contactin-4, an axonal glycoprotein belonging to the immunoglobulin superfamily, is expressed in a subpopulation of mouse olfactory sensory neurons. A mosaic pattern of glomerular arrangement is observed with strongly BIG-2-positive, weakly positive, and negative axon terminals in the olfactory bulb, which is overlapping but not identical with those of Kirrel2 and ephrin-A5. There is a close correlation between the BIG-2 expression level and the odorant receptor choice in individual sensory neurons. In BIG-2-deficient mice, olfactory sensory neurons expressing a given odorant receptor frequently innervate multiple glomeruli at ectopic locations. These results suggest that BIG-2 is one of the axon guidance molecules crucial for the formation and maintenance of functional odor map in the olfactory bulb.
AB - Olfactory sensory neurons expressing a given odorant receptor converge axons onto a few topographically fixed glomeruli in the olfactory bulb, leading to establishment of the odor map. Here, we report that BIG-2/contactin-4, an axonal glycoprotein belonging to the immunoglobulin superfamily, is expressed in a subpopulation of mouse olfactory sensory neurons. A mosaic pattern of glomerular arrangement is observed with strongly BIG-2-positive, weakly positive, and negative axon terminals in the olfactory bulb, which is overlapping but not identical with those of Kirrel2 and ephrin-A5. There is a close correlation between the BIG-2 expression level and the odorant receptor choice in individual sensory neurons. In BIG-2-deficient mice, olfactory sensory neurons expressing a given odorant receptor frequently innervate multiple glomeruli at ectopic locations. These results suggest that BIG-2 is one of the axon guidance molecules crucial for the formation and maintenance of functional odor map in the olfactory bulb.
KW - MOLNEURO
KW - SIGNALING
KW - SYSNEURO
UR - http://www.scopus.com/inward/record.url?scp=40849133914&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40849133914&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2008.01.023
DO - 10.1016/j.neuron.2008.01.023
M3 - Article
C2 - 18367085
AN - SCOPUS:40849133914
SN - 0896-6273
VL - 57
SP - 834
EP - 846
JO - Neuron
JF - Neuron
IS - 6
ER -