TY - JOUR
T1 - Biglycan and fibromodulin have essential roles in regulating chondrogenesis and extracellular matrix turnover in temporomandibular joint osteoarthritis
AU - Embree, Mildred C.
AU - Kilts, Tina M.
AU - Ono, Mitsuaki
AU - Inkson, Colette A.
AU - Syed-Picard, Fatima
AU - Karsdal, Morten A.
AU - Oldberg, Åke
AU - Bi, Yanming
AU - Young, Marian F.
N1 - Funding Information:
Supported in part by the Intramural Research Program of the NIH, NIDCR, and by an F-30 training grant to the Medical University of South Carolina from the Extramural Program of the NIH, NIDCR (to support M.E.).
PY - 2010/2
Y1 - 2010/2
N2 - The temporomandibular joint is critical for jaw movements and allows for mastication, digestion of food, and speech. Temporomandibular joint osteoarthritis is a degenerative disease that is marked by permanent cartilage destruction and loss of extracellular matrix (ECM). To understand how the ECM regulates mandibular condylar chondrocyte (MCC) differentiation and function, we used a genetic mouse model of temporomandibular joint osteoarthritis that is deficient in two ECM proteins, and fibromodulin (Bgn-/0 Fmod -/-). Given the unavailability of cell lines, we first isolated primary MCCs and found that they were phenotypically unique from hyaline articular chondrocytes isolated from the knee joint. Using Bgn -/0Fmod-/- MCCs, we discovered the early basis for temporomandibular joint osteoarthritis arises from abnormal and accelerated chondrogenesis. Transforming growth factor (TGF)-β1 is a growth factor that is critical for chondrogenesis and binds to both and fibromodulin. Our studies revealed the sequestration of TGF-β1 was decreased within the ECM of Bgn-/0Fmod-/- MCCs, leading to overactive TGF-β1 signal transduction. Using an explant culture system, we found that overactive TGF-β1 signals induced chondrogenesis and ECM turnover in this model. We demonstrated for the first time a comprehensive study revealing the importance of the ECM in maintaining the mandibular condylar cartilage integrity and identified biglycan and fibromodulin as novel key players in regulating chondrogenesis and ECM turnover during temoporomandibular joint osteoarthritis pathology.
AB - The temporomandibular joint is critical for jaw movements and allows for mastication, digestion of food, and speech. Temporomandibular joint osteoarthritis is a degenerative disease that is marked by permanent cartilage destruction and loss of extracellular matrix (ECM). To understand how the ECM regulates mandibular condylar chondrocyte (MCC) differentiation and function, we used a genetic mouse model of temporomandibular joint osteoarthritis that is deficient in two ECM proteins, and fibromodulin (Bgn-/0 Fmod -/-). Given the unavailability of cell lines, we first isolated primary MCCs and found that they were phenotypically unique from hyaline articular chondrocytes isolated from the knee joint. Using Bgn -/0Fmod-/- MCCs, we discovered the early basis for temporomandibular joint osteoarthritis arises from abnormal and accelerated chondrogenesis. Transforming growth factor (TGF)-β1 is a growth factor that is critical for chondrogenesis and binds to both and fibromodulin. Our studies revealed the sequestration of TGF-β1 was decreased within the ECM of Bgn-/0Fmod-/- MCCs, leading to overactive TGF-β1 signal transduction. Using an explant culture system, we found that overactive TGF-β1 signals induced chondrogenesis and ECM turnover in this model. We demonstrated for the first time a comprehensive study revealing the importance of the ECM in maintaining the mandibular condylar cartilage integrity and identified biglycan and fibromodulin as novel key players in regulating chondrogenesis and ECM turnover during temoporomandibular joint osteoarthritis pathology.
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U2 - 10.2353/ajpath.2010.090450
DO - 10.2353/ajpath.2010.090450
M3 - Article
C2 - 20035055
AN - SCOPUS:76149122505
SN - 0002-9440
VL - 176
SP - 812
EP - 826
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 2
ER -