TY - JOUR
T1 - Bile acids are new products of a marine bacterium, Myroides sp. strain SM1
AU - Maneerat, Suppasil
AU - Nitoda, Teruhiko
AU - Kanzaki, Hiroshi
AU - Kawai, Fusako
N1 - Funding Information:
Acknowledgements We are grateful to the JSPS-NRCT Core University Program for providing a scholarship to S.M., to The Nihonseimei Foundation for a grant to F.K. and to the Marine Biotechnology Institute, Iwate, Japan, for their kind supply of weathered crude oil. The authors express their thanks to the SC-NMR laboratory of Okayama University and the MS Laboratory of the Faculty of Agriculture, Okayama University, for assistance in the 1H-NMR and MS experiments, respectively
PY - 2005/6
Y1 - 2005/6
N2 - Strain SM1 was isolated as a biosurfactant-producing microorganism from seawater and presumptively identified as Myroides sp., based on morphology, biochemical characteristics and 16S rDNA sequence. The strain produced surface-active compounds in marine broth, which were purified, using emulsification activity for n-hexadecane as an indicator. The purified compounds were identified by thin-layer chromatography, 1H- and 13C-NMR spectra and fast atom bombardment mass spectrometry as cholic acid, deoxycholic acid and their glycine conjugates. Type strains of the genus Myroides, M. odoratus JCM7458 and M. odoramitimus JCM7460, also produced these compounds. Myroides sp. strain SM1 possessed a biosynthetic route to cholic acid from cholesterol. Thus, bile acids were found as new products of prokaryotic cells, genus Myroides.
AB - Strain SM1 was isolated as a biosurfactant-producing microorganism from seawater and presumptively identified as Myroides sp., based on morphology, biochemical characteristics and 16S rDNA sequence. The strain produced surface-active compounds in marine broth, which were purified, using emulsification activity for n-hexadecane as an indicator. The purified compounds were identified by thin-layer chromatography, 1H- and 13C-NMR spectra and fast atom bombardment mass spectrometry as cholic acid, deoxycholic acid and their glycine conjugates. Type strains of the genus Myroides, M. odoratus JCM7458 and M. odoramitimus JCM7460, also produced these compounds. Myroides sp. strain SM1 possessed a biosynthetic route to cholic acid from cholesterol. Thus, bile acids were found as new products of prokaryotic cells, genus Myroides.
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U2 - 10.1007/s00253-004-1777-1
DO - 10.1007/s00253-004-1777-1
M3 - Article
C2 - 15549287
AN - SCOPUS:21244487506
SN - 0175-7598
VL - 67
SP - 679
EP - 683
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 5
ER -
