Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

Shunji Hitotsubashi; Yoshio Fujii; Keinosuke Okamoto

doi:10.1111/j.1574-6968.1994.tb07183.x

Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

Shunji Hitotsubashi, Yoshio Fujii, Keinosuke Okamoto

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of ¹²⁵I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with ¹²⁵I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

Original language	English
Pages (from-to)	297-302
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	122
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1994.tb07183.x
Publication status	Published - Oct 1 1994
Externally published	Yes

Keywords

Escherichia coli
Heat-stable enterotoxin
Membrane protein
Receptor

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1994.tb07183.x

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title = "Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane",

abstract = "The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.",

keywords = "Escherichia coli, Heat-stable enterotoxin, Membrane protein, Receptor",

author = "Shunji Hitotsubashi and Yoshio Fujii and Keinosuke Okamoto",

note = "Funding Information: This study was supported in part by a grant from the Naito Foundation and by a grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture, Tokyo, Japan.",

year = "1994",

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doi = "10.1111/j.1574-6968.1994.tb07183.x",

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T1 - Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

AU - Hitotsubashi, Shunji

AU - Fujii, Yoshio

AU - Okamoto, Keinosuke

N1 - Funding Information: This study was supported in part by a grant from the Naito Foundation and by a grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture, Tokyo, Japan.

PY - 1994/10/1

Y1 - 1994/10/1

N2 - The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

AB - The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

KW - Escherichia coli

KW - Heat-stable enterotoxin

KW - Membrane protein

KW - Receptor

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JO - FEMS Microbiology Letters

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ER -

Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

Abstract

Keywords

ASJC Scopus subject areas

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