@article{2e1faf0284d14fc09dee85c2119098dd,
title = "Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes",
abstract = "Transformation with large DNA molecules enables multiple genes to be introduced into plants simultaneously to produce transgenic plants with complex phenotypes. In this study, a large DNA fragment (ca. 100 kb) containing a set of Aegilops tauschii hardness genes was introduced into rice plants using a novel transformation method, called bioactive beads-mediated transformation. Nine transgenic rice plants were obtained and the presence of transgenes in the rice genome was confirmed by PCR and FISH analyses. The results suggested that multiple transgenes were successfully integrated in all transgenic plants. The expression of one of the transgenes, puroindoline b, was confirmed at the mRNA and protein levels in the T2 generation. Our study clearly demonstrates that the bioactive bead method is capable of producing transgenic rice plants carrying large DNA fragments. This method will facilitate the production of useful transgenic plants by introducing multiple genes simultaneously.",
keywords = "Bioactive beads-mediated transformation, Hardness genes, Plant transformation, Rice, Transformation with large DNA fragment",
author = "Naoki Wada and Shin'Ichiro Kajiyama and Yukio Akiyama and Shigeki Kawakami and Daisuke No and Susumu Uchiyama and Motoyasu Otani and Takiko Shimada and Naoko Nose and Go Suzuki and Yasuhiko Mukai and Kiichi Fukui",
note = "Funding Information: Acknowledgments We are grateful to Dr. S. Rahman (CSIRO) for providing the BAC 10 and to D. No and Y. Murakami for technical assistance. We thank Dr. J.A. Cartagena (Osaka University) for helpful discussions and critically reading our manuscript. This research was supported by a fund from the Handai Frontier Research Center{\textquoteright}s Strategic Research Base supported by the Special Coordination Fund for Promoting Science and Technology of the Government of Japan to K. F. This research was partially supported by a Grant-in-Aid for Scientific Research (B) (No. 19380194) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We would also like to express special thanks to the Global COE (Center of Excellence) Program, {\textquoteleft}{\textquoteleft}Global Education and Research Center for Bio-Environmental Chemistry{\textquoteright}{\textquoteright}, of Osaka University. N.W. was supported by a Research Fellowship from the Japanese Society for the Promotion of Science for Young Scientists.",
year = "2009",
month = may,
doi = "10.1007/s00299-009-0678-2",
language = "English",
volume = "28",
pages = "759--768",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "5",
}