Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis

A. M. Ali, I. Intan-Safinar, M. M. Mackeen, S. H. El-Sharkawy, K. Takahata, H. Kanzaki, K. Kawazu

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62- melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines.

Original languageEnglish
Pages (from-to)180-183
Number of pages4
JournalNatural Product Sciences
Issue number3
Publication statusPublished - Nov 2 1998
Externally publishedYes


  • Cytotoxicity
  • Deoxypodophyllotoxin
  • Juniperus chinensis

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry


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