Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis

A. M. Ali; I. Intan-Safinar; M. M. Mackeen; S. H. El-Sharkawy; K. Takahata; H. Kanzaki; K. Kawazu

Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis

A. M. Ali, I. Intan-Safinar, M. M. Mackeen, S. H. El-Sharkawy, K. Takahata, H. Kanzaki, K. Kawazu

Research output: Contribution to journal › Article › peer-review

Abstract

The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62- melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines.

Original language	English
Pages (from-to)	180-183
Number of pages	4
Journal	Natural Product Sciences
Volume	4
Issue number	3
Publication status	Published - Nov 2 1998
Externally published	Yes

Keywords

Cytotoxicity
Deoxypodophyllotoxin
Juniperus chinensis

ASJC Scopus subject areas

Drug Discovery
Organic Chemistry

Cite this

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title = "Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis",

abstract = "The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62- melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines.",

keywords = "Cytotoxicity, Deoxypodophyllotoxin, Juniperus chinensis",

author = "Ali, {A. M.} and I. Intan-Safinar and Mackeen, {M. M.} and El-Sharkawy, {S. H.} and K. Takahata and H. Kanzaki and K. Kawazu",

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month = nov,

day = "2",

language = "English",

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journal = "Natural Product Sciences",

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T1 - Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis

AU - Ali, A. M.

AU - Intan-Safinar, I.

AU - Mackeen, M. M.

AU - El-Sharkawy, S. H.

AU - Takahata, K.

AU - Kanzaki, H.

AU - Kawazu, K.

PY - 1998/11/2

Y1 - 1998/11/2

N2 - The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62- melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines.

AB - The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62- melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines.

KW - Cytotoxicity

KW - Deoxypodophyllotoxin

KW - Juniperus chinensis

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AN - SCOPUS:0031726583

SN - 1226-3907

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JO - Natural Product Sciences

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ER -

Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis

Abstract

Keywords

ASJC Scopus subject areas

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