Biocompatibility of a Flowable Composite Bonded with a Self-etching Adhesive Compared with a Glass Ionomer Cement and a High Copper Amalgam

Y. Shimada, Y. Seki, Y. Sasafuchi, M. Arakawa, M. F. Burrow, M. Otsuki, J. Tagami

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6 Citations (Scopus)


This study evaluated the pulpal response and in-vivo microleakage of a flowable composite bonded with a self-etching adhesive and compared the results with a glass ionomer cement and amalgam. Cervical cavities were prepared in monkey teeth. The teeth were randomly divided into three groups. A self-etching primer system (Imperva FluoroBond, Shofu) was applied to the teeth in one of the experimental groups, and the cavities were filled with a flowable composite (SI-BF-2001-LF, Shofu). In the other groups, a glass ionomer cement (Fuji II, GC) or amalgam (Dispersalloy, Johnson & Johnson) filled the cavity. The teeth were then extracted after 3, 30 and 90 days, fixed in 10% buffered formalin solution and prepared according to routine histological techniques. Five micrometer sections were stained with hematoxylin and eosin or Brown and Brenn gram stain for bacterial observation. No serious inflammatory reaction of the pulp, such as necrosis or abscess formation, was observed in any of the experimental groups. Slight inflammatory cell infiltration was the main initial reaction, while deposition of reparative dentin was the major long-term reaction in all groups. No bacterial penetration along the cavity walls was detected in the flowable composite or glass ionomer cement except for one case at 30 days in the glass ionomer cement. The flowable composite bonded with self-etching adhesive showed an acceptable biological compatibility to monkey pulp. The in vivo sealing ability of the flowable composite in combination with the self-etching adhesive was considered comparable to glass ionomer cement. Amalgam restorations without adhesive liners showed slight bacterial penetration along the cavity wall.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalOperative dentistry
Issue number1
Publication statusPublished - Jan 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • General Dentistry


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