Biological control agent Rhizobium (=Agrobacterium) vitis strain ARK-1 suppresses expression of the essential and non-essential vir genes of tumorigenic R. vitis

Akira Kawaguchi; Mizuho Nita; Tomoya Ishii; Megumi Watanabe; Yoshiteru Noutoshi

doi:10.1186/s13104-018-4038-6

Biological control agent Rhizobium (=Agrobacterium) vitis strain ARK-1 suppresses expression of the essential and non-essential vir genes of tumorigenic R. vitis

Akira Kawaguchi, Mizuho Nita, Tomoya Ishii, Megumi Watanabe, Yoshiteru Noutoshi

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Objective: To gain insights into the virulence suppressive mechanism of a nonpathogenic strain of Rhizobium vitis ARK-1, we co-inoculated ARK-1 with a tumorigenic (Ti) strain of R. vitis to examine the expression of two essential virulence genes (virA and virG) and one non-essential gene (virD3) of the Ti strain at the wound site of grapevine. Results: Co-inoculation of ARK-1 with a Ti strain VAT03-9 at a 1:1 cell ratio into grapevine shoots resulted in significantly lower expression of the virulence genes virA, virD3, and virG of VAT03-9 at 1 day after inoculation compared with those when shoots were inoculated only with VAT03-9. ARK-1 was not able to catabolize acetosyringone, which is the plant-derived metabolites inducing the entire vir regulon in Ti strains, suggesting the direct effect of ARK-1 on the induction of broad range of vir genes of R. vitis Ti strains.

Original language	English
Article number	1
Journal	BMC Research Notes
Volume	12
Issue number	1
DOIs	https://doi.org/10.1186/s13104-018-4038-6
Publication status	Published - Jan 3 2019

Keywords

Acetosyringone
Biological control
Gene expression
Grapevine crown gall
Rhizobium vitis

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1186/s13104-018-4038-6

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title = "Biological control agent Rhizobium (=Agrobacterium) vitis strain ARK-1 suppresses expression of the essential and non-essential vir genes of tumorigenic R. vitis",

abstract = "Objective: To gain insights into the virulence suppressive mechanism of a nonpathogenic strain of Rhizobium vitis ARK-1, we co-inoculated ARK-1 with a tumorigenic (Ti) strain of R. vitis to examine the expression of two essential virulence genes (virA and virG) and one non-essential gene (virD3) of the Ti strain at the wound site of grapevine. Results: Co-inoculation of ARK-1 with a Ti strain VAT03-9 at a 1:1 cell ratio into grapevine shoots resulted in significantly lower expression of the virulence genes virA, virD3, and virG of VAT03-9 at 1 day after inoculation compared with those when shoots were inoculated only with VAT03-9. ARK-1 was not able to catabolize acetosyringone, which is the plant-derived metabolites inducing the entire vir regulon in Ti strains, suggesting the direct effect of ARK-1 on the induction of broad range of vir genes of R. vitis Ti strains.",

keywords = "Acetosyringone, Biological control, Gene expression, Grapevine crown gall, Rhizobium vitis",

author = "Akira Kawaguchi and Mizuho Nita and Tomoya Ishii and Megumi Watanabe and Yoshiteru Noutoshi",

note = "Funding Information: This research was supported by KAKENHI Grant 17H03778 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A. Kawaguchi and Y. Noutoshi. This funding worked in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. Publisher Copyright: {\textcopyright} 2019 The Author(s).",

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AU - Kawaguchi, Akira

AU - Nita, Mizuho

AU - Ishii, Tomoya

AU - Watanabe, Megumi

AU - Noutoshi, Yoshiteru

N1 - Funding Information: This research was supported by KAKENHI Grant 17H03778 from the Ministry of Education, Culture, Sports, Science and Technology of Japan to A. Kawaguchi and Y. Noutoshi. This funding worked in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. Publisher Copyright: © 2019 The Author(s).

PY - 2019/1/3

Y1 - 2019/1/3

N2 - Objective: To gain insights into the virulence suppressive mechanism of a nonpathogenic strain of Rhizobium vitis ARK-1, we co-inoculated ARK-1 with a tumorigenic (Ti) strain of R. vitis to examine the expression of two essential virulence genes (virA and virG) and one non-essential gene (virD3) of the Ti strain at the wound site of grapevine. Results: Co-inoculation of ARK-1 with a Ti strain VAT03-9 at a 1:1 cell ratio into grapevine shoots resulted in significantly lower expression of the virulence genes virA, virD3, and virG of VAT03-9 at 1 day after inoculation compared with those when shoots were inoculated only with VAT03-9. ARK-1 was not able to catabolize acetosyringone, which is the plant-derived metabolites inducing the entire vir regulon in Ti strains, suggesting the direct effect of ARK-1 on the induction of broad range of vir genes of R. vitis Ti strains.

AB - Objective: To gain insights into the virulence suppressive mechanism of a nonpathogenic strain of Rhizobium vitis ARK-1, we co-inoculated ARK-1 with a tumorigenic (Ti) strain of R. vitis to examine the expression of two essential virulence genes (virA and virG) and one non-essential gene (virD3) of the Ti strain at the wound site of grapevine. Results: Co-inoculation of ARK-1 with a Ti strain VAT03-9 at a 1:1 cell ratio into grapevine shoots resulted in significantly lower expression of the virulence genes virA, virD3, and virG of VAT03-9 at 1 day after inoculation compared with those when shoots were inoculated only with VAT03-9. ARK-1 was not able to catabolize acetosyringone, which is the plant-derived metabolites inducing the entire vir regulon in Ti strains, suggesting the direct effect of ARK-1 on the induction of broad range of vir genes of R. vitis Ti strains.

KW - Acetosyringone

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KW - Gene expression

KW - Grapevine crown gall

KW - Rhizobium vitis

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Biological control agent Rhizobium (=Agrobacterium) vitis strain ARK-1 suppresses expression of the essential and non-essential vir genes of tumorigenic R. vitis

Abstract

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