Biosynthetic study of amphidinolide B

M. Tsuda; T. Kubota; Y. Sakuma; J. Kobayashi

doi:10.1248/cpb.49.1366

Biosynthetic study of amphidinolide B

M. Tsuda, T. Kubota, Y. Sakuma, J. Kobayashi

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

The biosynthetic origins of amphidinolide B (1) were investigated on the basis of ¹³C-NMR data of ¹³C-enriched samples obtained by feeding experiments with [1-¹³C], [2-¹³C], and [1,2-¹³C₂] sodium acetates in cultures of a dinoflagellate Amphidinium sp. These incorporation patterns suggested that 1 was generated from three successive polyketide chains, an isolated C₁ unit from C-2 of acetates, six branched C₁ units from C-2 of acetates, and an "m-m" and an "m-m-m" unit derived only from C-2 of acetates. The labeling patterns of amphidinolide B (1) were different from those of amphidinolide H (2), a 26-membered macrolide closely related to 1.

Original language	English
Pages (from-to)	1366-1367
Number of pages	2
Journal	Chemical and Pharmaceutical Bulletin
Volume	49
Issue number	10
DOIs	https://doi.org/10.1248/cpb.49.1366
Publication status	Published - 2001
Externally published	Yes

Keywords

Biosynthesis
Macrolide
Marine dinoflagellate

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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10.1248/cpb.49.1366

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AU - Tsuda, M.

AU - Kubota, T.

AU - Sakuma, Y.

AU - Kobayashi, J.

PY - 2001

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AB - The biosynthetic origins of amphidinolide B (1) were investigated on the basis of 13C-NMR data of 13C-enriched samples obtained by feeding experiments with [1-13C], [2-13C], and [1,2-13C2] sodium acetates in cultures of a dinoflagellate Amphidinium sp. These incorporation patterns suggested that 1 was generated from three successive polyketide chains, an isolated C1 unit from C-2 of acetates, six branched C1 units from C-2 of acetates, and an "m-m" and an "m-m-m" unit derived only from C-2 of acetates. The labeling patterns of amphidinolide B (1) were different from those of amphidinolide H (2), a 26-membered macrolide closely related to 1.

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KW - Macrolide

KW - Marine dinoflagellate

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Biosynthetic study of amphidinolide B

Abstract

Keywords

ASJC Scopus subject areas

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