Caffeine inhalation effects on locomotor activity in mice

Hiroshi Ueno, Yu Takahashi, Shunsuke Suemitsu, Shinji Murakami, Naoya Kitamura, Kenta Wani, Yosuke Matsumoto, Motoi Okamoto, Takeshi Ishihara

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


It is estimated that 80% of the world’s population consumes caffeine from beverages and food every day. The traditional form of caffeine intake is oral, but more recently people have been inhaling caffeine using nasal sprays. However, the effects of caffeine inhalation are not well understood. The purpose of this study was to determine whether caffeine inhalation affects mouse behavior. To test this, we compared spontaneous activity of mice following inhalation and intraperitoneal administration of caffeine. Next, we investigated whether spontaneous activity changed with the time and/or concentration of caffeine inhaled. We found that mice that inhaled caffeine increased their spontaneous activity similar to mice that were administered caffeine intraperitoneally. Furthermore, spontaneous activity increased in an inhalation time-dependent and concentration-dependent manner. These results show that caffeine-induced stimulation also occurs by inhalation in mice, which suggests that caffeine can reach the brain even by inhalation. This study is useful not only for creating new administration methods of caffeine but also for adjusting caffeine storage and management.

Original languageEnglish
Pages (from-to)788-794
Number of pages7
JournalDrug Development and Industrial Pharmacy
Issue number5
Publication statusPublished - May 3 2020


  • Spontaneous activity
  • behavior
  • caffeine inhalation
  • locomotion
  • mouse

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry


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