TY - JOUR
T1 - Calcineurin Plays Different Roles in Group II Metabotropic Glutamate Receptor- and NMDA Receptor-Dependent Long-Term Depression
AU - Li, Sheng Tian
AU - Kato, Kunio
AU - Tomizawa, Kazuhito
AU - Matsushita, Masayuki
AU - Moriwaki, Akiyoshi
AU - Matsui, Hideki
AU - Mikoshiba, Katsuhiko
PY - 2002/6/15
Y1 - 2002/6/15
N2 - We investigated metabotropic glutamate receptor (mGluR)-dependent long-term depression (LTD) in hippocampal CA1 pyramidal neurons of 6- to 8-d-old [postnatal days 6-8 (P6-P8)] and 21 - to 25-d-old (P21-P25) rats. In P6-P8 rats, induction of LTD depended on the activity of group II mGluRs. In P21-P25 rats, however, this LTD disappeared, and instead, NMDA receptor (NMDAR)-dependent LTD appeared. A bath containing a specific calcineurin (CaN) inhibitor restored the group II mGluR-dependent LTD in the neurons of the P21-P25 rats. Although postsynaptic injection of CaN inhibitors suppressed NMDAR-dependent LTD, it did not affect induction of group II mGluR-dependent LTD. These results demonstrate that CaN plays different roles in the induction of two forms of LTD: presynaptic CaN inhibits group II mGluR-dependent LTD, whereas postsyn-aptic CaN facilitates NMDAR-dependent LTD. These findings are the first demonstration in vitro of group II mGluR-dependent LTD that is negatively regulated by CaN via an age-dependent mechanism.
AB - We investigated metabotropic glutamate receptor (mGluR)-dependent long-term depression (LTD) in hippocampal CA1 pyramidal neurons of 6- to 8-d-old [postnatal days 6-8 (P6-P8)] and 21 - to 25-d-old (P21-P25) rats. In P6-P8 rats, induction of LTD depended on the activity of group II mGluRs. In P21-P25 rats, however, this LTD disappeared, and instead, NMDA receptor (NMDAR)-dependent LTD appeared. A bath containing a specific calcineurin (CaN) inhibitor restored the group II mGluR-dependent LTD in the neurons of the P21-P25 rats. Although postsynaptic injection of CaN inhibitors suppressed NMDAR-dependent LTD, it did not affect induction of group II mGluR-dependent LTD. These results demonstrate that CaN plays different roles in the induction of two forms of LTD: presynaptic CaN inhibits group II mGluR-dependent LTD, whereas postsyn-aptic CaN facilitates NMDAR-dependent LTD. These findings are the first demonstration in vitro of group II mGluR-dependent LTD that is negatively regulated by CaN via an age-dependent mechanism.
KW - Hippocampus
KW - LTD
KW - Long-term potentiation
KW - MGluR
KW - NMDA receptor
KW - Synaptic plasticity
UR - http://www.scopus.com/inward/record.url?scp=0037096282&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037096282&partnerID=8YFLogxK
U2 - 10.1523/jneurosci.22-12-05034.2002
DO - 10.1523/jneurosci.22-12-05034.2002
M3 - Article
C2 - 12077199
AN - SCOPUS:0037096282
SN - 0270-6474
VL - 22
SP - 5034
EP - 5041
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 12
ER -