Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds

Lamia Sakouhi, Sondès Rahoui, Marouane Ben Massoud, Shintaro Munemasa, Ezzeddine EL Ferjani, Yoshiyuki Murata, Abdelilah Chaoui

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Impact of exogenous calcium and ethylene glycol tetraacetic acid (EGTA) supplement on chickpea (Cicer arietinum L.) germinating seeds exposed to cadmium stress for 6 days was studied. Ca and EGTA late treatment (3 days) alleviated growth inhibition and decreased Cd accumulation as well as lipid peroxidation and protein carbonylation in both root and shoot cells. Exogenous effector application relieved Cd-induced cell death which was associated with a constant level of ATP, which was considered as an apoptotic-like process. Redox balance was examined through the study of the redox state of pyridine nucleotide couples NAD+/NADH and NADP+/NADPH as well as their related oxidative [NAD(P)H-oxidase] and dehydrogenase (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malate dehydrogenase) enzyme activities. The present research illustrated an ameliorative effect of Ca and EGTA on growth of Cd-exposed chickpea seedlings that occurs through the protection of sensitive cell sites from Cd-induced oxidation, namely membrane lipids and proteins, rather than the improvement of recycling capabilities of the cellular reducing power.

Original languageEnglish
Pages (from-to)1064-1073
Number of pages10
JournalJournal of Plant Growth Regulation
Issue number4
Publication statusPublished - Dec 1 2016


  • Cadmium
  • Calcium
  • Chickpea
  • EGTA
  • Stress alleviation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


Dive into the research topics of 'Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds'. Together they form a unique fingerprint.

Cite this