TY - JOUR
T1 - Caries increment and salivary microbiome during university life
T2 - A prospective cohort study
AU - Uchida-Fukuhara, Yoko
AU - Ekuni, Daisuke
AU - Islam, Md Monirul
AU - Kataoka, Kota
AU - Tabata, Ayano
AU - Fukuhara, Daiki
AU - Toyama, Naoki
AU - Kobayashi, Terumasa
AU - Fujimori, Kohei
AU - Sawada, Nanami
AU - Iwasaki, Yoshiaki
AU - Morita, Manabu
N1 - Funding Information:
Funding: This work was financially supported by a Grant-in-Aid for Scientific Research (no. 15K11415) from the Ministry of Education, Culture, Sports, Science, and Technology, Tokyo, Japan.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/5/2
Y1 - 2020/5/2
N2 - The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (∆decayed, missing, and filled teeth (∆DMFT) score increased during the 3-year period) and non-increased group (∆DMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group (n = 14) was similar to that in the non-increased group (n = 41). Relative abundances of the family Prevotellaceae (p = 0.007) and genera Alloprevotella (p = 0.007) and Dialister (p = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.
AB - The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (∆decayed, missing, and filled teeth (∆DMFT) score increased during the 3-year period) and non-increased group (∆DMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group (n = 14) was similar to that in the non-increased group (n = 41). Relative abundances of the family Prevotellaceae (p = 0.007) and genera Alloprevotella (p = 0.007) and Dialister (p = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.
KW - Dental caries
KW - Oral health
KW - Saliva
KW - Salivary microbiome
KW - Sequence analysis
KW - Young adult
UR - http://www.scopus.com/inward/record.url?scp=85085602460&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85085602460&partnerID=8YFLogxK
U2 - 10.3390/ijerph17103713
DO - 10.3390/ijerph17103713
M3 - Article
C2 - 32466124
AN - SCOPUS:85085602460
SN - 1661-7827
VL - 17
JO - International Journal of Environmental Research and Public Health
JF - International Journal of Environmental Research and Public Health
IS - 10
M1 - 3713
ER -
