TY - JOUR
T1 - Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray
AU - Utsumi, Yoshinori
AU - Tanaka, Maho
AU - Kurotani, Atsushi
AU - Yoshida, Takuhiro
AU - Mochida, Keiichi
AU - Matsui, Akihiro
AU - Ishitani, Manabu
AU - Sraphet, Supajit
AU - Whankaew, Sukhuman
AU - Asvarak, Thipa
AU - Narangajavana, Jarunya
AU - Triwitayakorn, Kanokporn
AU - Sakurai, Tetsuya
AU - Seki, Motoaki
N1 - Funding Information:
This work was supported by the Japan Science and Technology Agency (JST) and the National Science and Technology Development Agency of Thailand (Grant No.P12-01382) under the East Asia Science and Innovation Area Joint Research Program (e-ASIA JRP). This research was also supported by the Strategic Funds for the Promotion of Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; RIKEN Center for Sustainable Resource Science (CSRS); and CREST, JST, Japan. JN and KT were supported by the National Science and Technology Development Agency, Thailand, (Grant No. P-12-01382), under the East Asia Science and Innovation Area Joint Research Program (e-ASIA JRP).
Publisher Copyright:
© 2016, The Botanical Society of Japan and Springer Japan.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Cassava anthracnose disease (CAD), caused by the fungus Colletotrichumgloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tags and RNA-seq information from cassava, and applied it to investigate the molecular mechanisms of resistance to fungal infection using two cassava cultivars, Huay Bong 60 (HB60, resistant to CAD) and Hanatee (HN, sensitive to CAD). Based on quantitative real-time reverse transcription PCR and expression profiling by the microarray, we showed that the expressions of various plant defense-related genes, such as pathogenesis-related (PR) genes, cell wall-related genes, detoxification enzyme, genes related to the response to bacterium, mitogen-activated protein kinase (MAPK), genes related to salicylic acid, jasmonic acid and ethylene pathways were higher in HB60 compared with HN. Our results indicated that the induction of PR genes in HB60 by fungal infection and the higher expressions of defense response-related genes in HB60 compared with HN are likely responsible for the fungal resistance in HB60. We also showed that the use of our cassava oligo microarray could improve our understanding of cassava molecular mechanisms related to environmental responses and development, and advance the molecular breeding of useful cassava plants.
AB - Cassava anthracnose disease (CAD), caused by the fungus Colletotrichumgloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tags and RNA-seq information from cassava, and applied it to investigate the molecular mechanisms of resistance to fungal infection using two cassava cultivars, Huay Bong 60 (HB60, resistant to CAD) and Hanatee (HN, sensitive to CAD). Based on quantitative real-time reverse transcription PCR and expression profiling by the microarray, we showed that the expressions of various plant defense-related genes, such as pathogenesis-related (PR) genes, cell wall-related genes, detoxification enzyme, genes related to the response to bacterium, mitogen-activated protein kinase (MAPK), genes related to salicylic acid, jasmonic acid and ethylene pathways were higher in HB60 compared with HN. Our results indicated that the induction of PR genes in HB60 by fungal infection and the higher expressions of defense response-related genes in HB60 compared with HN are likely responsible for the fungal resistance in HB60. We also showed that the use of our cassava oligo microarray could improve our understanding of cassava molecular mechanisms related to environmental responses and development, and advance the molecular breeding of useful cassava plants.
KW - Cassava
KW - Cassava anthracnose disease
KW - Detoxification
KW - Disease resistance
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=84965069051&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84965069051&partnerID=8YFLogxK
U2 - 10.1007/s10265-016-0828-x
DO - 10.1007/s10265-016-0828-x
M3 - Article
C2 - 27138000
AN - SCOPUS:84965069051
SN - 0918-9440
VL - 129
SP - 711
EP - 726
JO - Journal of Plant Research
JF - Journal of Plant Research
IS - 4
ER -