TY - JOUR
T1 - Catalases negatively regulate methyl jasmonate signaling in guard cells
AU - Jannat, Rayhanur
AU - Uraji, Misugi
AU - Hossain, Mohammad Anowar
AU - Islam, Mohammad Muzahidul
AU - Nakamura, Yoshimasa
AU - Mori, Izumi C.
AU - Murata, Yoshiyuki
N1 - Funding Information:
This research was supported in part by Grant-in-Aid for Young Scientists and Grants for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Nissan Science Foundation , the Ohara Foundation for Agricultural Research and Special Account Budget for Education and Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan .
PY - 2012/7/1
Y1 - 2012/7/1
N2 - Methyl jasmonate (MeJA)-induced stomatal closure is accompanied by the accumulation of hydrogen peroxide (H 2O 2) in guard cells. In this study, we investigated the roles of catalases (CATs) in MeJA-induced stomatal closure using cat mutants cat2, cat3-1 and cat1 cat3, and the CAT inhibitor, 3-aminotriazole (AT). When assessed with 2',7'-dichlorodihydrofluorescein, the reduction of catalase activity by means of mutations and the inhibitor accumulated higher basal levels of H 2O 2 in guard cells whereas they did not affect stomatal aperture in the absence of MeJA. In contrast, the cat mutations and the treatment with AT potentiated MeJA-induced stomatal closure and MeJA-induced H 2O 2 production. These results indicate that CATs negatively regulate H 2O 2 accumulation in guard cells and suggest that inducible H 2O 2 production rather than constitutive elevation modulates stomatal apertures in Arabidopsis.
AB - Methyl jasmonate (MeJA)-induced stomatal closure is accompanied by the accumulation of hydrogen peroxide (H 2O 2) in guard cells. In this study, we investigated the roles of catalases (CATs) in MeJA-induced stomatal closure using cat mutants cat2, cat3-1 and cat1 cat3, and the CAT inhibitor, 3-aminotriazole (AT). When assessed with 2',7'-dichlorodihydrofluorescein, the reduction of catalase activity by means of mutations and the inhibitor accumulated higher basal levels of H 2O 2 in guard cells whereas they did not affect stomatal aperture in the absence of MeJA. In contrast, the cat mutations and the treatment with AT potentiated MeJA-induced stomatal closure and MeJA-induced H 2O 2 production. These results indicate that CATs negatively regulate H 2O 2 accumulation in guard cells and suggest that inducible H 2O 2 production rather than constitutive elevation modulates stomatal apertures in Arabidopsis.
KW - Catalase
KW - Guard cells
KW - Hydrogen peroxide
KW - Methyl jasmonate
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U2 - 10.1016/j.jplph.2012.03.006
DO - 10.1016/j.jplph.2012.03.006
M3 - Article
C2 - 22525681
AN - SCOPUS:84861773864
SN - 0176-1617
VL - 169
SP - 1012
EP - 1016
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 10
ER -