TY - JOUR
T1 - Caveolin-1 as tumor suppressor gene in breast cancer
AU - Hino, Masato
AU - Doihara, Hiroyoshi
AU - Kobayashi, Kazuyasu
AU - Aoe, Motoi
AU - Shimizu, Nobuyoshi
PY - 2003
Y1 - 2003
N2 - Purpose. Many articles have reported the caveolin-1 gene to be downregulated thus suggesting that it might be a candidate tumor suppressor gene in many tumors, but in bladder tumors the caveolin-1 gene was expressed and related to the pathological grade. We investigated the function of caveolin-1 protein in breast tumors. Methods. We introduced the caveolin-1 gene into a MCF-7 human breast cancer cell line, and examined its cell growth, cell viability, and anchorage-independent growth. Results. The caveolin-1 transfectants showed less proliferation than the vector control transfectants on the fourth day regarding the cell growth rate. In addition, the cell viability of the caveolin-1 transfectants was about 55 % of the vector control. A soft agar assay of the caveolin-1 transfectants showed less growth with a lower number of colonies. Conclusion. In MCF-7 cells, the caveolin-1 gene may influence the tumor suppressor efficacy.
AB - Purpose. Many articles have reported the caveolin-1 gene to be downregulated thus suggesting that it might be a candidate tumor suppressor gene in many tumors, but in bladder tumors the caveolin-1 gene was expressed and related to the pathological grade. We investigated the function of caveolin-1 protein in breast tumors. Methods. We introduced the caveolin-1 gene into a MCF-7 human breast cancer cell line, and examined its cell growth, cell viability, and anchorage-independent growth. Results. The caveolin-1 transfectants showed less proliferation than the vector control transfectants on the fourth day regarding the cell growth rate. In addition, the cell viability of the caveolin-1 transfectants was about 55 % of the vector control. A soft agar assay of the caveolin-1 transfectants showed less growth with a lower number of colonies. Conclusion. In MCF-7 cells, the caveolin-1 gene may influence the tumor suppressor efficacy.
KW - Caveolin-1
KW - Cell growth
KW - MCF-7
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U2 - 10.1007/s10595-002-2538-4
DO - 10.1007/s10595-002-2538-4
M3 - Article
C2 - 14506991
AN - SCOPUS:0038353161
SN - 0941-1291
VL - 33
SP - 486
EP - 490
JO - Japanese Journal of Surgery
JF - Japanese Journal of Surgery
IS - 7
ER -
