Cell-based fabrication of organic/inorganic composite gel material

Takuya Matsumoto, Ami Mizuno, Miki Kashiwagi, Shin Suke Yoshida, Jun Ichi Sasaki, Takayoshi Nakano

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Biomaterials containing components similar to the native biological tissue would have benefits as an implantable scaffold material. To obtain such biomimetic materials, cells may be great contributors because of their crucial roles in synthetic organics. In addition, the synthesized organics-especially those derived from osteogenic differentiated cells-become a place where mineral crystals nucleate and grow even in vitro. Therefore, to fabricate an organic/inorganic composite material, which is similar to the biological osteoid tissue, bone marrow derived mesenchymal stem cells (BMSCs) were cultured in a 3D fibrin gel in this study. BMSCs secreted bone-related proteins that enhanced the biomineralization within the gel when the cells were cultured with an osteogenic differentiation medium. The compositions of both synthesized matrices and precipitated minerals in the obtained materials altered depending on the cell culture period. The mineral obtained in the 3D gel showed low crystalline hydroxyapatite. The composite materials also showed excellent osteoconductivity with new bone formation when implanted in mice tibiae. Thus, we demonstrated the contributions of cells for fabricating implantable organic/inorganic composite gel materials and a method for controlling the material composition in the gel. This cell-based material fabrication method would be a novel method to fabricate organic/inorganic composite biomimetic materials for bone tissue engineering.

Original languageEnglish
Pages (from-to)327-338
Number of pages12
Issue number1
Publication statusPublished - 2010
Externally publishedYes


  • 3D
  • Biomimetic material
  • Hydrogel
  • Organic/inorganic composite

ASJC Scopus subject areas

  • Condensed Matter Physics
  • General Materials Science


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