Cell spreading on titanium dioxide film formed and modified with aerosol beam and femtosecond laser

Togo Shinonaga, Masahiro Tsukamoto, Akiko Nagai, Kimihiro Yamashita, Takao Hanawa, Nobuhiro Matsushita, Guoqiang Xie, Nobuyuki Abe

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Titanium (Ti) is widely used in biomaterials because of its excellent anti-corrosion properties and high strength. However, Ti has no biological function, so its bioactivity must be improved. Coating a titanium dioxide (TiO 2 ) film on a Ti plate surface has been shown to improve the biocompatibility of Ti plates. If periodic nanostructures were formed on the film surface, the direction of cell spreading might be controlled by the direction of the grooves. Controlling cell spreading on biomaterials would contribute to the creation of advanced biomaterials. In this paper, a TiO 2 film was formed on a Ti plate with an aerosol beam composed of sub micron-sized TiO 2 particles and helium gas. Periodic nanostructures, lying perpendicular to the laser electric field polarization vector, were formed on the film by scanning the femtosecond laser focusing spot. The period and height of the periodic nanostructures were about 230 nm and 150 nm, respectively. In a cell test, cell spreading was observed along the grooves of the periodic nanostructures; in contrast, cell spreading did not show a definite direction on TiO 2 a film without periodic nanostructures. These results suggest that the direction of cell spreading on the film can be controlled by periodic nanostructure formation generated using a femtosecond laser.

Original languageEnglish
Pages (from-to)649-653
Number of pages5
JournalApplied Surface Science
Publication statusPublished - Jan 1 2014
Externally publishedYes


  • Aerosol beam
  • Cell spreading
  • Femtosecond laser
  • Periodic nanostructures
  • Titanium dioxide film

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films


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