Centrally administered ghrelin activates cardiac vagal nerve in anesthetized rabbits

Shuji Shimizu; Tsuyoshi Akiyama; Toru Kawada; Takashi Sonobe; Atsunori Kamiya; Toshiaki Shishido; Takeshi Tokudome; Hiroshi Hosoda; Mikiyasu Shirai; Kenji Kangawa; Masaru Sugimachi

doi:10.1016/j.autneu.2011.04.001

Centrally administered ghrelin activates cardiac vagal nerve in anesthetized rabbits

Shuji Shimizu, Tsuyoshi Akiyama, Toru Kawada, Takashi Sonobe, Atsunori Kamiya, Toshiaki Shishido, Takeshi Tokudome, Hiroshi Hosoda, Mikiyasu Shirai, Kenji Kangawa, Masaru Sugimachi

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Although central ghrelin has cardioprotective effect through inhibiting sympathetic nerve activity, the effects of central ghrelin on cardiac vagal nerve remain unknown. We investigated the effects of centrally administered ghrelin on cardiac autonomic nerve activities using microdialysis technique. A microdialysis probe was implanted in the right atrial wall adjacent to the sinoatrial node of an anesthetized rabbit and was perfused with Ringer's solution containing a cholinesterase inhibitor, eserine. After injection of ghrelin (1. nmol) into the right lateral cerebral ventricle, norepinephrine (NE) and acetylcholine (ACh) concentrations in the dialysate samples were measured as indices of NE and ACh release from nerve endings to the sinoatrial node using high-performance liquid chromatography. Heart rate was 270 ± 4. bpm at baseline and decreased gradually after ghrelin injection to 234 ± 9. bpm (P < 0.01) at 60-80. min, followed by gradual recovery. Dialysate ACh concentration was 5.5 ± 0.8. nM at baseline and increased gradually after ghrelin injection to 8.8 ± 1.2. nM (P < 0.01) at 60-80. min; the concentration started to decrease gradually from 100 to 120. min after injection reaching 5.6 ± 0.8 nM at 160-180. min. Central ghrelin did not change mean arterial pressure or dialysate NE concentration. The elevated dialysate ACh concentration declined rapidly after transection of cervical vagal nerves. These results indicate that centrally administered ghrelin activates cardiac vagal nerve.

Original language	English
Pages (from-to)	60-65
Number of pages	6
Journal	Autonomic Neuroscience: Basic and Clinical
Volume	162
Issue number	1-2
DOIs	https://doi.org/10.1016/j.autneu.2011.04.001
Publication status	Published - Jul 5 2011
Externally published	Yes

Keywords

Acetylcholine
Cardiac microdialysis
Ghrelin
Norepinephrine
Vagal nerve

ASJC Scopus subject areas

Endocrine and Autonomic Systems
Clinical Neurology
Cellular and Molecular Neuroscience

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10.1016/j.autneu.2011.04.001

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@article{b3da252d0ace4e4b8f90fca9fa8e26f5,

title = "Centrally administered ghrelin activates cardiac vagal nerve in anesthetized rabbits",

abstract = "Although central ghrelin has cardioprotective effect through inhibiting sympathetic nerve activity, the effects of central ghrelin on cardiac vagal nerve remain unknown. We investigated the effects of centrally administered ghrelin on cardiac autonomic nerve activities using microdialysis technique. A microdialysis probe was implanted in the right atrial wall adjacent to the sinoatrial node of an anesthetized rabbit and was perfused with Ringer's solution containing a cholinesterase inhibitor, eserine. After injection of ghrelin (1. nmol) into the right lateral cerebral ventricle, norepinephrine (NE) and acetylcholine (ACh) concentrations in the dialysate samples were measured as indices of NE and ACh release from nerve endings to the sinoatrial node using high-performance liquid chromatography. Heart rate was 270 ± 4. bpm at baseline and decreased gradually after ghrelin injection to 234 ± 9. bpm (P < 0.01) at 60-80. min, followed by gradual recovery. Dialysate ACh concentration was 5.5 ± 0.8. nM at baseline and increased gradually after ghrelin injection to 8.8 ± 1.2. nM (P < 0.01) at 60-80. min; the concentration started to decrease gradually from 100 to 120. min after injection reaching 5.6 ± 0.8 nM at 160-180. min. Central ghrelin did not change mean arterial pressure or dialysate NE concentration. The elevated dialysate ACh concentration declined rapidly after transection of cervical vagal nerves. These results indicate that centrally administered ghrelin activates cardiac vagal nerve.",

keywords = "Acetylcholine, Cardiac microdialysis, Ghrelin, Norepinephrine, Vagal nerve",

author = "Shuji Shimizu and Tsuyoshi Akiyama and Toru Kawada and Takashi Sonobe and Atsunori Kamiya and Toshiaki Shishido and Takeshi Tokudome and Hiroshi Hosoda and Mikiyasu Shirai and Kenji Kangawa and Masaru Sugimachi",

note = "Funding Information: This study was supported by the research project promoted by Ministry of Health, Labour and Welfare in Japan (H20-katsudo-Shitei-007, H21-nano-ippan-005), the Grants-in-Aid for Scientific Research promoted by Ministry of Education, Culture, Sports, Science and Technology in Japan (# 20390462 and # 20590242 ) and the Industrial Technology Research Grant Program from New Energy and Industrial Technology Development Organization (NEDO) of Japan .",

year = "2011",

month = jul,

day = "5",

doi = "10.1016/j.autneu.2011.04.001",

language = "English",

volume = "162",

pages = "60--65",

journal = "Autonomic Neuroscience: Basic and Clinical",

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T1 - Centrally administered ghrelin activates cardiac vagal nerve in anesthetized rabbits

AU - Shimizu, Shuji

AU - Akiyama, Tsuyoshi

AU - Kawada, Toru

AU - Sonobe, Takashi

AU - Kamiya, Atsunori

AU - Shishido, Toshiaki

AU - Tokudome, Takeshi

AU - Hosoda, Hiroshi

AU - Shirai, Mikiyasu

AU - Kangawa, Kenji

AU - Sugimachi, Masaru

N1 - Funding Information: This study was supported by the research project promoted by Ministry of Health, Labour and Welfare in Japan (H20-katsudo-Shitei-007, H21-nano-ippan-005), the Grants-in-Aid for Scientific Research promoted by Ministry of Education, Culture, Sports, Science and Technology in Japan (# 20390462 and # 20590242 ) and the Industrial Technology Research Grant Program from New Energy and Industrial Technology Development Organization (NEDO) of Japan .

PY - 2011/7/5

Y1 - 2011/7/5

N2 - Although central ghrelin has cardioprotective effect through inhibiting sympathetic nerve activity, the effects of central ghrelin on cardiac vagal nerve remain unknown. We investigated the effects of centrally administered ghrelin on cardiac autonomic nerve activities using microdialysis technique. A microdialysis probe was implanted in the right atrial wall adjacent to the sinoatrial node of an anesthetized rabbit and was perfused with Ringer's solution containing a cholinesterase inhibitor, eserine. After injection of ghrelin (1. nmol) into the right lateral cerebral ventricle, norepinephrine (NE) and acetylcholine (ACh) concentrations in the dialysate samples were measured as indices of NE and ACh release from nerve endings to the sinoatrial node using high-performance liquid chromatography. Heart rate was 270 ± 4. bpm at baseline and decreased gradually after ghrelin injection to 234 ± 9. bpm (P < 0.01) at 60-80. min, followed by gradual recovery. Dialysate ACh concentration was 5.5 ± 0.8. nM at baseline and increased gradually after ghrelin injection to 8.8 ± 1.2. nM (P < 0.01) at 60-80. min; the concentration started to decrease gradually from 100 to 120. min after injection reaching 5.6 ± 0.8 nM at 160-180. min. Central ghrelin did not change mean arterial pressure or dialysate NE concentration. The elevated dialysate ACh concentration declined rapidly after transection of cervical vagal nerves. These results indicate that centrally administered ghrelin activates cardiac vagal nerve.

AB - Although central ghrelin has cardioprotective effect through inhibiting sympathetic nerve activity, the effects of central ghrelin on cardiac vagal nerve remain unknown. We investigated the effects of centrally administered ghrelin on cardiac autonomic nerve activities using microdialysis technique. A microdialysis probe was implanted in the right atrial wall adjacent to the sinoatrial node of an anesthetized rabbit and was perfused with Ringer's solution containing a cholinesterase inhibitor, eserine. After injection of ghrelin (1. nmol) into the right lateral cerebral ventricle, norepinephrine (NE) and acetylcholine (ACh) concentrations in the dialysate samples were measured as indices of NE and ACh release from nerve endings to the sinoatrial node using high-performance liquid chromatography. Heart rate was 270 ± 4. bpm at baseline and decreased gradually after ghrelin injection to 234 ± 9. bpm (P < 0.01) at 60-80. min, followed by gradual recovery. Dialysate ACh concentration was 5.5 ± 0.8. nM at baseline and increased gradually after ghrelin injection to 8.8 ± 1.2. nM (P < 0.01) at 60-80. min; the concentration started to decrease gradually from 100 to 120. min after injection reaching 5.6 ± 0.8 nM at 160-180. min. Central ghrelin did not change mean arterial pressure or dialysate NE concentration. The elevated dialysate ACh concentration declined rapidly after transection of cervical vagal nerves. These results indicate that centrally administered ghrelin activates cardiac vagal nerve.

KW - Acetylcholine

KW - Cardiac microdialysis

KW - Ghrelin

KW - Norepinephrine

KW - Vagal nerve

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IS - 1-2

ER -

Centrally administered ghrelin activates cardiac vagal nerve in anesthetized rabbits

Abstract

Keywords

ASJC Scopus subject areas

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