Change of concentrations of trace elements and protein contents in the liver of zinc deficient mice

R. Minayoshi; T. Ohyama; N. Kinugawa; J. Kamishima; T. Ogi; K. Ishikawa; M. Noguchi; H. Suganuma; K. Takahashi; S. Enomoto; M. Yanaga

doi:10.1007/s10967-007-0541-2

Change of concentrations of trace elements and protein contents in the liver of zinc deficient mice

R. Minayoshi, T. Ohyama, N. Kinugawa, J. Kamishima, T. Ogi, K. Ishikawa, M. Noguchi, H. Suganuma, K. Takahashi, S. Enomoto, M. Yanaga

Research output: Contribution to journal › Article › peer-review

Abstract

The concentrations of essential trace elements and proteins in cytosolic fraction of hepatic cells of mice fed with Zn-deficient diet (Zn-def, mice) and control were determined by ICP-MS and BCA protein assay method, respectively, after division into forty fractions by gel filtration chromatography. The concentrations of zinc and proteins decreased in the 14-17th fractions of Zn-def, mice, whereas cobalt concentrations increased in the 14, 17, and 18th fractions. However, no significant differences were found on the gel after SDS-PAGE for the 12-21st fractions, although the BCA protein assay date showed the decrease of protein amounts in 13-15th fractions of Zn-def. mice.

Original language	English
Pages (from-to)	429-431
Number of pages	3
Journal	Journal of Radioanalytical and Nuclear Chemistry
Volume	272
Issue number	2
DOIs	https://doi.org/10.1007/s10967-007-0541-2
Publication status	Published - May 2007
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Nuclear Energy and Engineering
Radiology Nuclear Medicine and imaging
Pollution
Spectroscopy
Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10967-007-0541-2

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title = "Change of concentrations of trace elements and protein contents in the liver of zinc deficient mice",

abstract = "The concentrations of essential trace elements and proteins in cytosolic fraction of hepatic cells of mice fed with Zn-deficient diet (Zn-def, mice) and control were determined by ICP-MS and BCA protein assay method, respectively, after division into forty fractions by gel filtration chromatography. The concentrations of zinc and proteins decreased in the 14-17th fractions of Zn-def, mice, whereas cobalt concentrations increased in the 14, 17, and 18th fractions. However, no significant differences were found on the gel after SDS-PAGE for the 12-21st fractions, although the BCA protein assay date showed the decrease of protein amounts in 13-15th fractions of Zn-def. mice.",

author = "R. Minayoshi and T. Ohyama and N. Kinugawa and J. Kamishima and T. Ogi and K. Ishikawa and M. Noguchi and H. Suganuma and K. Takahashi and S. Enomoto and M. Yanaga",

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doi = "10.1007/s10967-007-0541-2",

language = "English",

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T1 - Change of concentrations of trace elements and protein contents in the liver of zinc deficient mice

AU - Minayoshi, R.

AU - Ohyama, T.

AU - Kinugawa, N.

AU - Kamishima, J.

AU - Ogi, T.

AU - Ishikawa, K.

AU - Noguchi, M.

AU - Suganuma, H.

AU - Takahashi, K.

AU - Enomoto, S.

AU - Yanaga, M.

PY - 2007/5

Y1 - 2007/5

N2 - The concentrations of essential trace elements and proteins in cytosolic fraction of hepatic cells of mice fed with Zn-deficient diet (Zn-def, mice) and control were determined by ICP-MS and BCA protein assay method, respectively, after division into forty fractions by gel filtration chromatography. The concentrations of zinc and proteins decreased in the 14-17th fractions of Zn-def, mice, whereas cobalt concentrations increased in the 14, 17, and 18th fractions. However, no significant differences were found on the gel after SDS-PAGE for the 12-21st fractions, although the BCA protein assay date showed the decrease of protein amounts in 13-15th fractions of Zn-def. mice.

AB - The concentrations of essential trace elements and proteins in cytosolic fraction of hepatic cells of mice fed with Zn-deficient diet (Zn-def, mice) and control were determined by ICP-MS and BCA protein assay method, respectively, after division into forty fractions by gel filtration chromatography. The concentrations of zinc and proteins decreased in the 14-17th fractions of Zn-def, mice, whereas cobalt concentrations increased in the 14, 17, and 18th fractions. However, no significant differences were found on the gel after SDS-PAGE for the 12-21st fractions, although the BCA protein assay date showed the decrease of protein amounts in 13-15th fractions of Zn-def. mice.

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Change of concentrations of trace elements and protein contents in the liver of zinc deficient mice

Abstract

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