Changes in CD4+CD8-/CD4-CD8+ ratio and humoral immune functions in vitamin B12-deficient rats

U. Funada, M. Wada, T. Kawata, K. Mori, H. Tamai, T. Kawanishi, A. Kunou, N. Tanaka, T. Tadokoro, A. Maekawa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


To clarify the role of vitamin B12 in the function of cell-mediated and humoral immune functions, the splenocytes expression of CD4, CD8 and serum C3, IgM, IgG concentrations were examined in vitamin B12-deficient rats, and the effect of the administration of methylcobalamin was also studied. The CD4+CD8-/CD4-CD8+ ratio in splenocytes was significantly higher in vitamin B12-deficient rats than in control rats (p < 0.05). The value in the 48 hours after methylcobalamin administration group, was within the normal range (p < 0.05). From these results, the elevation of the CD4+CD8-/CD4-CD8+ ratio by vitamin B12-deficiency was confirmed in rats. The serum C3, IgM and IgG concentrations were lower in the vitamin B12-deficient group than in the control group. These findings suggest that vitamin B12 plays a role in maintaining the immune function in rats.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalInternational Journal for Vitamin and Nutrition Research
Issue number4
Publication statusPublished - Jan 1 2000
Externally publishedYes


  • CD4
  • CD8
  • Serum C3
  • Serum IgG
  • Serum IgM
  • Vitamin B-deficiency

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics


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