Changes in metallothionein isoform expression in the bones of ovariectomized rats

Chiharu Sogawa; Mika Ikegame; Ikuko Miyazaki; Toshiaki Ara; Yasuhiro Imamura; Yuka Okusha; Kazumi Ohyama; Masato Asanuma; Norio Sogawa; Toshio Yamamoto; Ken-ichi Kozaｋi

doi:10.2485/jhtb.25.21

Changes in metallothionein isoform expression in the bones of ovariectomized rats

Chiharu Sogawa, Mika Ikegame, Ikuko Miyazaki, Toshiaki Ara, Yasuhiro Imamura, Yuka Okusha, Kazumi Ohyama, Masato Asanuma, Norio Sogawa, Toshio Yamamoto, Ken-ichi Kozaｋi

Research output: Contribution to journal › Article › peer-review

Abstract

Metal-binding proteins, metallothioneins (MTs), may play important roles in bone metabolism. However, the contribution of MTs to bone metabolism remains obscure. In the present study, we investigated the expression of MT isoforms in bone cells and mRNA for MT isoforms in the tibiae following ovariectomy (OVX). The results obtained showed that MT-I/II and MT-III were expressed in osteoblasts, osteoclasts, and osteocytes 4 weeks after OVX. Peaks in the mRNA expression ratios (OVX/Sham) of MT-I/II and MT-III changed following OVX. The expression ratio of MT-I/II increased after 1 week, whereas that of MT-III increased 4 weeks after OVX. These results suggest that the contribution of MTs to bone metabolism may depend on the isoforms in the cell types and the stage after OVX.

Original language	English
Pages (from-to)	21-26
Number of pages	6
Journal	Journal of Hard Tissue Biology
Volume	25
Issue number	1
DOIs	https://doi.org/10.2485/jhtb.25.21
Publication status	Published - Jan 15 2016

Keywords

Metallothionein
Odontoblast
Osteoclast
Osteocyte
Ovariectomy

ASJC Scopus subject areas

Medicine (miscellaneous)
Biochemistry
Biomaterials
Orthopedics and Sports Medicine
Dentistry(all)
Cell Biology

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10.2485/jhtb.25.21

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title = "Changes in metallothionein isoform expression in the bones of ovariectomized rats",

abstract = "Metal-binding proteins, metallothioneins (MTs), may play important roles in bone metabolism. However, the contribution of MTs to bone metabolism remains obscure. In the present study, we investigated the expression of MT isoforms in bone cells and mRNA for MT isoforms in the tibiae following ovariectomy (OVX). The results obtained showed that MT-I/II and MT-III were expressed in osteoblasts, osteoclasts, and osteocytes 4 weeks after OVX. Peaks in the mRNA expression ratios (OVX/Sham) of MT-I/II and MT-III changed following OVX. The expression ratio of MT-I/II increased after 1 week, whereas that of MT-III increased 4 weeks after OVX. These results suggest that the contribution of MTs to bone metabolism may depend on the isoforms in the cell types and the stage after OVX.",

keywords = "Metallothionein, Odontoblast, Osteoclast, Osteocyte, Ovariectomy",

author = "Chiharu Sogawa and Mika Ikegame and Ikuko Miyazaki and Toshiaki Ara and Yasuhiro Imamura and Yuka Okusha and Kazumi Ohyama and Masato Asanuma and Norio Sogawa and Toshio Yamamoto and Ken-ichi Kozaｋi",

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doi = "10.2485/jhtb.25.21",

language = "English",

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T1 - Changes in metallothionein isoform expression in the bones of ovariectomized rats

AU - Sogawa, Chiharu

AU - Ikegame, Mika

AU - Miyazaki, Ikuko

AU - Ara, Toshiaki

AU - Imamura, Yasuhiro

AU - Okusha, Yuka

AU - Ohyama, Kazumi

AU - Asanuma, Masato

AU - Sogawa, Norio

AU - Yamamoto, Toshio

AU - Kozaｋi, Ken-ichi

PY - 2016/1/15

Y1 - 2016/1/15

N2 - Metal-binding proteins, metallothioneins (MTs), may play important roles in bone metabolism. However, the contribution of MTs to bone metabolism remains obscure. In the present study, we investigated the expression of MT isoforms in bone cells and mRNA for MT isoforms in the tibiae following ovariectomy (OVX). The results obtained showed that MT-I/II and MT-III were expressed in osteoblasts, osteoclasts, and osteocytes 4 weeks after OVX. Peaks in the mRNA expression ratios (OVX/Sham) of MT-I/II and MT-III changed following OVX. The expression ratio of MT-I/II increased after 1 week, whereas that of MT-III increased 4 weeks after OVX. These results suggest that the contribution of MTs to bone metabolism may depend on the isoforms in the cell types and the stage after OVX.

AB - Metal-binding proteins, metallothioneins (MTs), may play important roles in bone metabolism. However, the contribution of MTs to bone metabolism remains obscure. In the present study, we investigated the expression of MT isoforms in bone cells and mRNA for MT isoforms in the tibiae following ovariectomy (OVX). The results obtained showed that MT-I/II and MT-III were expressed in osteoblasts, osteoclasts, and osteocytes 4 weeks after OVX. Peaks in the mRNA expression ratios (OVX/Sham) of MT-I/II and MT-III changed following OVX. The expression ratio of MT-I/II increased after 1 week, whereas that of MT-III increased 4 weeks after OVX. These results suggest that the contribution of MTs to bone metabolism may depend on the isoforms in the cell types and the stage after OVX.

KW - Metallothionein

KW - Odontoblast

KW - Osteoclast

KW - Osteocyte

KW - Ovariectomy

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Changes in metallothionein isoform expression in the bones of ovariectomized rats

Abstract

Keywords

ASJC Scopus subject areas

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