Characterization of a CENP-C homolog in Arabidopsis thaliana

Yutaka Ogura; Fukashi Shibata; Hiroshi Sato; Minoru Murata

doi:10.1266/ggs.79.139

Characterization of a CENP-C homolog in Arabidopsis thaliana

Yutaka Ogura, Fukashi Shibata, Hiroshi Sato, Minoru Murata

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Genes and Genetic Systems
Volume	79
Issue number	3
DOIs	https://doi.org/10.1266/ggs.79.139
Publication status	Published - Jun 2004
Externally published	Yes

Keywords

180-bp repeat
Arabidopsis thaliana
CENP-C
Centromere
Immunofluorescence

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1266/ggs.79.139

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abstract = "Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.",

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AU - Ogura, Yutaka

AU - Shibata, Fukashi

AU - Sato, Hiroshi

AU - Murata, Minoru

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KW - Immunofluorescence

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Characterization of a CENP-C homolog in Arabidopsis thaliana

Abstract

Keywords

ASJC Scopus subject areas

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