Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: A Dethiobiotin producing Bacterium

Yoshikazu Izumi; Yoshiaki Kano; Kenji Inagaki; Naoki Kawase; Yoshiki Tani; Hideaki Yamada

doi:10.1271/bbb1961.45.1983

Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: A Dethiobiotin producing Bacterium

Yoshikazu Izumi, Yoshiaki Kano, Kenji Inagaki, Naoki Kawase, Yoshiki Tani, Hideaki Yamada

Research output: Contribution to journal › Article › peer-review

Abstract

A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined. Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

Original language	English
Pages (from-to)	1983-1989
Number of pages	7
Journal	Agricultural and Biological Chemistry
Volume	45
Issue number	9
DOIs	https://doi.org/10.1271/bbb1961.45.1983
Publication status	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1271/bbb1961.45.1983

Cite this

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title = "Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: A Dethiobiotin producing Bacterium",

abstract = "A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined. Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.",

author = "Yoshikazu Izumi and Yoshiaki Kano and Kenji Inagaki and Naoki Kawase and Yoshiki Tani and Hideaki Yamada",

year = "1981",

doi = "10.1271/bbb1961.45.1983",

language = "English",

volume = "45",

pages = "1983--1989",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "9",

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T1 - Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus

T2 - A Dethiobiotin producing Bacterium

AU - Izumi, Yoshikazu

AU - Kano, Yoshiaki

AU - Inagaki, Kenji

AU - Kawase, Naoki

AU - Tani, Yoshiki

AU - Yamada, Hideaki

PY - 1981

Y1 - 1981

N2 - A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined. Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

AB - A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined. Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

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DO - 10.1271/bbb1961.45.1983

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SN - 0916-8451

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 9

ER -

Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: A Dethiobiotin producing Bacterium

Abstract

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