Characterization of exopolymeric substances from selected Rhodopseudomonas palustris strains and their ability to adsorb sodium ions

Tomorn Nunkaew; Duangporn Kantachote; Teruhiko Nitoda; Hiroshi Kanzaki; Raymond J. Ritchie

doi:10.1016/j.carbpol.2014.08.099

Characterization of exopolymeric substances from selected Rhodopseudomonas palustris strains and their ability to adsorb sodium ions

Tomorn Nunkaew, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki, Raymond J. Ritchie

School of Agriculture

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Removal of Na⁺by binding with exopolymeric substances (EPS) from Rhodopseudomonas palustris TN114 and PP803 was investigated. The moderate negative correlation pairs (r_p) between remaining Alcian blue and amount of Na⁺adsorbed on EPS from strains TN114 and PP803 were -0.652 and -0.609. Both strains showed positive relationships between the amounts of EPS produced and bacterial growth. EPS from strain PP803 had a higher efficiency in removing Na⁺than the EPS from strain TN114 based on their EC₅₀values (1.79 and 1.49 mg/mL for TN114 and PP803, respectively). The principal component from EPS of strain PP803 which was responsible for salt removal was purified and it was identified as a polysaccharide (≈18 kDa) mainly composed of galacturonic acid. Overall results suggested that EPS is a key factor that our strains used to bind Na⁺allowing their survival in high NaCl concentrations.

Original language	English
Pages (from-to)	334-341
Number of pages	8
Journal	Carbohydrate Polymers
Volume	115
DOIs	https://doi.org/10.1016/j.carbpol.2014.08.099
Publication status	Published - Jan 22 2014

Keywords

Biofertilizers
Exopolymeric substances
Galacturonic acid
Rhodopseudomonas palustris
Rice
Salinity

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.carbpol.2014.08.099

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title = "Characterization of exopolymeric substances from selected Rhodopseudomonas palustris strains and their ability to adsorb sodium ions",

abstract = "Removal of Na+by binding with exopolymeric substances (EPS) from Rhodopseudomonas palustris TN114 and PP803 was investigated. The moderate negative correlation pairs (rp) between remaining Alcian blue and amount of Na+adsorbed on EPS from strains TN114 and PP803 were -0.652 and -0.609. Both strains showed positive relationships between the amounts of EPS produced and bacterial growth. EPS from strain PP803 had a higher efficiency in removing Na+than the EPS from strain TN114 based on their EC50values (1.79 and 1.49 mg/mL for TN114 and PP803, respectively). The principal component from EPS of strain PP803 which was responsible for salt removal was purified and it was identified as a polysaccharide (≈18 kDa) mainly composed of galacturonic acid. Overall results suggested that EPS is a key factor that our strains used to bind Na+allowing their survival in high NaCl concentrations.",

keywords = "Biofertilizers, Exopolymeric substances, Galacturonic acid, Rhodopseudomonas palustris, Rice, Salinity",

author = "Tomorn Nunkaew and Duangporn Kantachote and Teruhiko Nitoda and Hiroshi Kanzaki and Ritchie, {Raymond J.}",

note = "Funding Information: This work was financial supported by Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program; grant number PHD/0200/2551 , and partly supported by Graduate School, Prince of Songkla University . We thank Dr. Brian Hodgson for his English assistance. Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.carbpol.2014.08.099",

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AU - Nunkaew, Tomorn

AU - Kantachote, Duangporn

AU - Nitoda, Teruhiko

AU - Kanzaki, Hiroshi

AU - Ritchie, Raymond J.

N1 - Funding Information: This work was financial supported by Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program; grant number PHD/0200/2551 , and partly supported by Graduate School, Prince of Songkla University . We thank Dr. Brian Hodgson for his English assistance. Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 2014/1/22

Y1 - 2014/1/22

N2 - Removal of Na+by binding with exopolymeric substances (EPS) from Rhodopseudomonas palustris TN114 and PP803 was investigated. The moderate negative correlation pairs (rp) between remaining Alcian blue and amount of Na+adsorbed on EPS from strains TN114 and PP803 were -0.652 and -0.609. Both strains showed positive relationships between the amounts of EPS produced and bacterial growth. EPS from strain PP803 had a higher efficiency in removing Na+than the EPS from strain TN114 based on their EC50values (1.79 and 1.49 mg/mL for TN114 and PP803, respectively). The principal component from EPS of strain PP803 which was responsible for salt removal was purified and it was identified as a polysaccharide (≈18 kDa) mainly composed of galacturonic acid. Overall results suggested that EPS is a key factor that our strains used to bind Na+allowing their survival in high NaCl concentrations.

AB - Removal of Na+by binding with exopolymeric substances (EPS) from Rhodopseudomonas palustris TN114 and PP803 was investigated. The moderate negative correlation pairs (rp) between remaining Alcian blue and amount of Na+adsorbed on EPS from strains TN114 and PP803 were -0.652 and -0.609. Both strains showed positive relationships between the amounts of EPS produced and bacterial growth. EPS from strain PP803 had a higher efficiency in removing Na+than the EPS from strain TN114 based on their EC50values (1.79 and 1.49 mg/mL for TN114 and PP803, respectively). The principal component from EPS of strain PP803 which was responsible for salt removal was purified and it was identified as a polysaccharide (≈18 kDa) mainly composed of galacturonic acid. Overall results suggested that EPS is a key factor that our strains used to bind Na+allowing their survival in high NaCl concentrations.

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KW - Salinity

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Characterization of exopolymeric substances from selected Rhodopseudomonas palustris strains and their ability to adsorb sodium ions

Abstract

Keywords

ASJC Scopus subject areas

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